Ca2+ sparks and Ca2+ waves in saponin-permeabilized rat ventricular myocytes
Ca2+ sparks and Ca2+ waves in saponin-permeabilized rat ventricular myocytes
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DOI：
10.1111/j.1469-7793.1999.00575.x
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发表时间：
1999-12-15
影响因子：
5.5
通讯作者：
Györke, S
中科院分区：
文献类型：
--
作者：
Lukyanenko, V;Györke, S
1.我们在含有MgATP、EGTA和fluo-3钾盐的“内部”溶液中用皂苷透化的心肌细胞进行了共聚焦Ca 2+成像。透化的心肌细胞表现出自发的Ca 2+火花和波类似于在加载fluo-3 AM的完整心肌细胞中观察到的那些。在“低”[EGTA]（0.05 mM）的存在下，Ca 2+波有规律地出现，即使在相对低的[Ca 2 +]（50-100 nM，游离）。增加[EGTA]导致Ca 2+波的频率和传播速度降低。在[EGTA] > 0.3 mM时，重复波完全消失。火花的频率作为[Ca 2 +]（50-400 nM范围）的函数增加，没有高亲和力Ca 2+依赖性失活过程的迹象。钙调素和环腺苷二磷酸核糖（cADPR）可增加钙火花的发生率。
1. We carried out confocal Ca2+ imaging in myocytes permeabilized with saponin in 'internal' solutions containing: MgATP, EGTA and fluo-3 potassium salt.2. Permeabilized myocytes exhibited spontaneous Ca2+ sparks and waves similar to those observed in intact myocytes loaded with fluo-3 AM.3. In the presence of 'low' [EGTA] (0.05 mM), Ca2+ waves arose regularly, even at relatively low [Ca2+] (50-100 nM, free). Increasing [EGTA] resulted in decreased frequency and propagation velocity of Ca2+ waves. Propagating waves were completely abolished at [EGTA] > 0.3 mM.4. The frequency of sparks increased as a function of [Ca2+] (50-400 nM range) with no sign of a high affinity Ca2+-dependent inactivation process.5. The rate of occurrence of Ca2+ sparks was increased by calmodulin and cyclic adenosine diphosphate-ribose (cADPR).