RNASE P-ACTIVITY IN THE MITOCHONDRIA OF SACCHAROMYCES-CEREVISIAE DEPENDS ON BOTH MITOCHONDRION AND NUCLEUS-ENCODED COMPONENTS
RNASE P-ACTIVITY IN THE MITOCHONDRIA OF SACCHAROMYCES-CEREVISIAE DEPENDS ON BOTH MITOCHONDRION AND NUCLEUS-ENCODED COMPONENTS
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DOI：
10.1128/mcb.6.4.1058
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发表时间：
1986-04-01
影响因子：
5.3
通讯作者：
MARTIN, NC
中科院分区：
文献类型：
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作者：
HOLLINGSWORTH, MJ;MARTIN, NC
TRNA生物合成的一个必要步骤是从tRNA前体中去除5‘’前导序列。我们已经在酵母线粒体提取物中检测到RNaseP活性，它可以在同源前体tRNA上进行这一反应。这种线粒体核糖核酸酶P对微球菌核酸酶和蛋白酶都敏感，表明它的活性既需要核酸，也需要蛋白质。RNase P在体外活性的存在与酵母线粒体DNA上存在一个基因座直接相关，此前的遗传和生化研究表明，tRNA成熟需要一个基因座。该位点的产物9S RNA与新描述的线粒体RNase P活性共分离，进一步证明9S RNA是酵母线粒体RNase P的RNA组分。
A requisite step in the biosynthesis of tRNA is the removal of 5'' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA. This mitochondrial RNase P was sensitive to both micrococcal nuclease and protease, demonstrating that it requires both a nucleic acid and protein for activity. The presence of RNase P activity in vitro directly correlated with the presence of a locus on yeast mitochondrial DNA previously shown by genetic and biochemical studies to be required for tRNA maturation. The product of the locus, the 9S RNA, and this newly described mitochondrial RNase P activity cofractionated, providing further evidence that the 9S RNA is the RNA component of yeast mitochondrial RNase P.