Imprint of human cytomegalovirus infection on the NK cell receptor repertoire
Imprint of human cytomegalovirus infection on the NK cell receptor repertoire
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DOI：
10.1182/blood-2004-05-2058
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发表时间：
2004-12-01
期刊：
影响因子：
20.3
通讯作者：
López-Botet, M
中科院分区：
文献类型：
--
作者：
Gumá, M;Angulo, A;López-Botet, M
分析了健康成人献血者外周血淋巴细胞（PBL）中HLA-E特异性活化CD 94/NKG 2C杀伤凝集素样受体（KLR）的表达;还研究了其他自然杀伤（NK）细胞受体（即，CD 94/NKG 2A、KIR、CD 85 j、CD 161、NKp 46、NKp 30和NKG 2D）的表达。人巨细胞病毒（HCMV）感染以及HLA-E和杀伤免疫球蛋白样受体（KIR）基因型被认为是与CD 94/NKG 2C表达相关的潜在相关变量。NKG 2C（+）淋巴细胞的比例在很宽的范围内变化（< 0.1%至22.1%），并且注意到NKG 2C + NK和T细胞之间的显著相关性（r = 0.83; P <0.001）。供者的HLA-E基因型和激活KIR基因的数目与NKG 2C+淋巴细胞的百分比无显著相关性。相比之下，HCMV血清学阳性，而不是其他疱疹病毒（即EB病毒和单纯疱疹病毒），与NKG 2C + NK和T细胞比例增加密切相关（P <0.001）。值得注意的是，与CD 94/NKG 2A（+）细胞相比，CD 94/NKG 2C（+）细胞群表达的天然细胞毒性受体（NCR）（即NKp 30、NKp 46）水平较低，并且包含的KIR+和CD 85 j（+）细胞比例较高。总之，这些数据支持HCMV感染选择性地塑造来自健康携带者个体的NK和T细胞的自然杀伤细胞受体（NKR）库。(C)2004年，美国血液学会。
Expression of the activating CD94/NKG2C killer lectin-like receptor (KLR) specific for HLA-E was analyzed in peripheral blood lymphocytes (PBLs) from healthy adult blood donors; the expression of other natural killer (NK) cell receptors (ie, CD94/NKG2A, KIR, CD85j, CD161, NKp46, NKp30, and NKG2D) was also studied. Human cytomegalovirus (HCMV) infection as well as the HLA-E and killer immunoglobulin-like receptor (KIR) genotypes were considered as potentially relevant variables associated with CD94/NKG2C expression. The proportion of NKG2C(+) lymphocytes varied within a wide range ( < 0.1% to 22.1%), and a significant correlation (r = 0.83; P < .001) between NKG2C+ NK and T cells was noticed. The HLA-E genotype and the number of activating KIR genes of the donors were not significantly related to the percentage of NKG2C+ lymphocytes. By contrast, a positive serology for HCMV, but not for other herpesviruses (ie, Epstein-Barr and herpes simplex), turned out to be strongly associated (P < .001) with increased proportions of NKG2C+ NK and T cells. Remarkably, the CD94/NKG2C(+) population expressed lower levels of natural cytotoxicity receptors (NCRs) (ie, NKp30, NKp46) and included higher proportions of KIR+ and CD85j(+) cells than CD94/NKG2A(+) cells. Altogether, these data support that HCMV Infection selectively shapes the natural killer cell receptor (NKR) repertoire of NK and T cells from healthy carrier Individuals. (C) 2004 by The American Society of Hematology.