Functional expression and germline transmission of a human chromosome fragment in chimaeric mice
Functional expression and germline transmission of a human chromosome fragment in chimaeric mice
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DOI：
10.1038/ng0697-133
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发表时间：
1997-06-01
期刊：
影响因子：
30.8
通讯作者：
Ishida, I
中科院分区：
文献类型：
--
作者：
Tomizuka, K;Yoshida, H;Ishida, I
将人染色体或来源于正常成纤维细胞的染色体片段通过微细胞介导的染色体转移(MMCT)导入小鼠胚胎干细胞，并从中获得可存活的嵌合体小鼠。转移的染色体被稳定地保留，人类基因，包括免疫球蛋白(Ig)kappa、Heavy、lambda基因，在成人嵌合组织中以适当的组织特异性方式表达。在人类染色体(hChr.)2衍生片段的情况下，发现它是通过生殖系传递给后代的。我们的研究表明，MMCT允许将非常大量的外来遗传物质引入小鼠体内。这一新的程序将有助于在活体内对人类基因组进行功能分析。
Human chromosomes or chromosome fragments derived from normal fibroblasts were introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Transferred chromosomes were stably retained, and human genes, including immunoglobulin (Ig) kappa, heavy, lambda genes, were expressed in proper tissue-specific manner in adult chimaeric tissues. In the case of a human chromosome (hChr.) 2-derived fragment, it was found to be transmitted to the offspring through the germline. Our study demonstrates that MMCT allows for introduction of very large amounts of foreign genetic material into mice. This novel procedure will facilitate the functional analyses of human genomes in vivo.