PaACL silencing accelerates flower senescence and changes the proteome to maintain metabolic homeostasis in Petunia hybrida.
PaACL silencing accelerates flower senescence and changes the proteome to maintain metabolic homeostasis in Petunia hybrida.
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PaACL 沉默会加速花朵衰老并改变蛋白质组以维持矮牵牛的代谢稳态。
DOI：
10.1093/jxb/eraa208
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发表时间：
2020
影响因子：
6.9
通讯作者：
Yu Yixun
中科院分区：
文献类型：
--
作者：
Zhao Huina;Zhong Shiwei;Sang Lina;Zhang Xinyou;Chen Zeyu;Wei Qian;Chen Guoju;Liu Juanxu;Yu Yixun
摘要胞浆乙酰辅酶A是大多数次生代谢产物合成的中间体，也是蛋白质乙酰化的乙酰基来源。由柠檬酸盐形成细胞溶质乙酰辅酶A由ATP-柠檬酸盐裂解酶（ACL）催化。然而，ACL在整体代谢物合成和整体蛋白质乙酰化中的功能尚不清楚。在这里，四个基因，PaACLA 1，PaACLA 2，PaACLB 1，和PaACLB 2，编码的ACLA和ACLB亚基的腋花矮牵牛，被确定为相同的序列在矮牵牛杂交'超级'。PaACLA 1-A2和PaACLB 1-B2基因的沉默导致矮牵牛“Ultra”叶片和花发育异常，总花色素苷含量降低，花衰老加速。代谢组和乙酰组分析表明，PaACLB 1-B2沉默增加了乙酰辅酶A代谢的许多下游代谢产物的含量和许多蛋白质的乙酰化水平在矮牵牛花冠。从机制上讲，在PaACL沉默的矮牵牛花冠乙酰辅酶A减少诱导的代谢应激引起转录组，蛋白质组和乙酰组的全球和具体的变化，维持代谢稳态的影响。此外，乙酰辅酶A缺乏时，整体蛋白质组与乙酰组呈负相关。总之，我们的研究结果表明，ACL作为一个重要的代谢调节器，通过促进转录组，蛋白质组的变化，维持代谢稳态。和乙酰组。
Abstract Cytosolic acetyl-CoA is an intermediate of the synthesis of most secondary metabolites and the source of acetyl for protein acetylation. The formation of cytosolic acetyl-CoA from citrate is catalysed by ATP-citrate lyase (ACL). However, the function of ACL in global metabolite synthesis and global protein acetylation is not well known. Here, four genes, PaACLA1, PaACLA2, PaACLB1, and PaACLB2, which encode the ACLA and ACLB subunits of ACL in Petunia axillaris, were identified as the same sequences in Petunia hybrida ‘Ultra’. Silencing of PaACLA1-A2 and PaACLB1-B2 led to abnormal leaf and flower development, reduced total anthocyanin content, and accelerated flower senescence in petunia ‘Ultra’. Metabolome and acetylome analysis revealed that PaACLB1-B2 silencing increased the content of many downstream metabolites of acetyl-CoA metabolism and the levels of acetylation of many proteins in petunia corollas. Mechanistically, the metabolic stress induced by reduction of acetyl-CoA in PaACL-silenced petunia corollas caused global and specific changes in the transcriptome, the proteome, and the acetylome, with the effect of maintaining metabolic homeostasis. In addition, the global proteome and acetylome were negatively correlated under acetyl-CoA deficiency. Together, our results suggest that ACL acts as an important metabolic regulator that maintains metabolic homeostasis by promoting changes in the transcriptome, proteome. and acetylome.