Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants
Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants
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DOI：
10.1063/1.2458162
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发表时间：
2007-02-12
影响因子：
4
通讯作者：
Kong, M. G.
中科院分区：
文献类型：
--
作者：
Perni, Stefano;Shama, Gilbert;Kong, M. G.
等离子体诱导的微生物灭活机制通常采用物理化学技术进行研究。在这封信中，大肠杆菌K-12和它的三角洲recA，三角洲rpoS，和三角洲soxS突变体被用来区分紫外光子，OH自由基，和活性氧物种在大气放电中产生的影响。这种微生物学方法利用了这些E.大肠杆菌突变体对各种外部胁迫的抗性是有缺陷的。通过相互作用的细菌灭活动力学与光学发射光谱，氧原子被确定为一个主要的贡献者，在血浆灭活与紫外光子，OH自由基，单线态氧可降解物，和一氧化氮的次要贡献。(c)2007年，美国物理学会。
Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its Delta recA, Delta rpoS, and Delta soxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. (c) 2007 American Institute of Physics.