Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing
Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing
复制标题
通过碱基编辑在水稻中产生对 ALS 抑制性除草剂的广谱耐受性
DOI：
10.1007/s11427-020-1800-5
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发表时间：
2020-11-03
影响因子：
9.1
通讯作者：
Gao, Caixia
中科院分区：
文献类型：
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作者：
Zhang, Rui;Chen, Sha;Gao, Caixia
通过基因组编辑产生的耐除草剂水稻品种对于杂草控制是非常理想的。我们使用胞嘧啶碱基编辑器在乙酰乳酸合成酶（ALS）基因的P171和/或G628密码子中创建了一系列错义突变，以赋予水稻除草剂耐受性。P171密码子中的四种不同错义突变P171 S、P171 A、P171 Y和P171 F对来自ALS抑制剂的五个化学家族的五种代表性除草剂表现出不同的耐受模式。例如，P171 S和P171 A对双草醚的耐受性低于P171 Y和P171 F，但对其他除草剂的耐受性不低于P171 Y和P171 F。有趣的是，一种新的三重突变体（P171 F/G628 E/G629 S）对所有五种测试的除草剂具有最高的耐受性。田间试验表明，P171 F和P171 F/G628 E/G629 S都有可能与烟嘧磺隆一起使用。我们的工作说明了一种使用碱基编辑在优质水稻品种中产生除草剂耐受性的有效方法。
Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control. We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of theacetolactate synthase(ALS) gene to confer herbicide tolerance in rice. The four different missense mutations in the P171 codon, P171S, P171A, P171Y and P171F, exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors. For example, P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides. Interestingly, a novel triple mutant (P171F/G628E/G629S) had the highest tolerance to all five tested herbicides. Field trials showed that both P171F and P171F/G628E/G629S could potentially be used with nicosulfuron. Our work illustrates an effective way of using base editing to generate herbicide tolerance in elite rice varieties.