Reduction of enterotoxigenic Escherichia coli colonization by the oral administration of Lactobacillus casei as a probiotic in a murine model
Reduction of enterotoxigenic Escherichia coli colonization by the oral administration of Lactobacillus casei as a probiotic in a murine model
复制标题
DOI：
--
复制
发表时间：
2010-11
影响因子：
--
通讯作者：
R. Mirnejad;J. Hossein;Abdolla Ardebilli;H. Babavalian
中科院分区：
文献类型：
--
作者：
R. Mirnejad;J. Hossein;Abdolla Ardebilli;H. Babavalian
本研究的目的是在小鼠模型中检查通过口服干酪乳杆菌作为益生菌减少肠致病性大肠杆菌（ETEC）定植的情况。在这项研究中，36只BALB/c小鼠被分为两个试验组和一个对照组。其中一个试验组在第六天喂食益生菌，其他组在第三天喂食益生菌;而对照组不接受任何益生菌。在最后一次口服益生菌后72 h，所有三个组均喂食ETEC。E.用菌落计数法测定了不同时间动物肠道排泄的大肠杆菌在特定培养基上的定殖率。本研究结果表明，L.酪蛋白能够在小鼠胃肠道中定殖，并且与对照组相比，两个试验组的小鼠显示出ETEC排泄显著降低（P = 0.001）。此外，测试组的比较表明，益生菌的较长时间接收导致病原菌定殖的更多减少。这些结果提示L.酪蛋白可用作候选益生菌，以降低ETEC在消化道中的定殖和建立速率，并预防由该生物体引起的腹泻。关键词：干酪乳杆菌GG株，大肠埃希菌，益生菌，肠源性旅行者腹泻，细菌治疗
The aim of this study was to examine the reduction of enterotoxigenic Escherichia coli (ETEC) colonization by oral administration of Lactobacillus casei as probiotic in a murine model. In this study, thirty-six BALB/c mice were divided to two test groups and one control group. One of the test groups was fed probiotic bacteria at six days and other groups at three days; whereas, the control group did not receive any probiotic. 72 h after the last oral administration of probiotic, the all three groups were fed by the ETEC. Than, the numbers of E. coli excreted from animal intestinal were determined at various times by colony counting on specific culture media and assessed their rate of colonization. The results of this study showed that L. casei is enable to be colonize in the murine gastrointestinal tract and both test groups of murine show significant decrease of ETEC excretion compared to control group (P = 0.001). Also, comparison of test groups indicated that longer time reception of probiotic bacteria resulted in more reduction of colonization of pathogenic bacteria. These findings suggest that L. caseican be used as a candidate probiotic to reduce the rate of colonization and establishment of ETEC in the digestive tract and to prevent diarrhea caused by this organism. Key words: Lactobacillus casei sp. strain GG, Escherichia coli, probiotic, enterotoxigenic traveler's diarrhea, bacteriotherapy