The genome of the protist parasite Entamoeba histolytica
The genome of the protist parasite Entamoeba histolytica
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DOI：
10.1038/nature03291
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发表时间：
2005-02-24
期刊：
影响因子：
64.8
通讯作者：
Hall, N
中科院分区：
文献类型：
--
作者：
Loftus, B;Anderson, I;Hall, N
溶组织内阿米巴是一种肠道寄生虫，是阿米巴病的病原体，是发展中国家发病率和死亡率的重要来源(1)。在这里，我们展示了溶组织棘球绦虫的基因组，它揭示了与另外两种线粒体原生生物病原体：蓝氏贾第鞭毛虫和阴道毛滴虫共享的各种代谢适应。这些适应包括减少或消除大多数线粒体代谢途径，以及使用通常与厌氧原核生物相关的氧化应激酶。系统基因组学分析确定了细菌基因横向转移到溶组织埃希菌基因组中的证据，其影响集中在溶组织埃希菌代谢谱的扩展方面。这些基因的存在和在溶组织埃希菌中新的代谢途径的可能性可能允许新的化疗药物的开发。基因组编码大量新的受体激酶，并包含各种基因家族的扩展，包括那些与毒力相关的基因。基因组的其他特征包括大量重复的含有转移RNA的阵列，这些阵列可能在基因组中具有结构功能。对基因组的分析为人类主要病原体的工作原理和基因组进化提供了新的见解。
Entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries(1). Here we present the genome of E. histolytica, which reveals a variety of metabolic adaptations shared with two other amitochondrial protist pathogens: Giardia lamblia and Trichomonas vaginalis. These adaptations include reduction or elimination of most mitochondrial metabolic pathways and the use of oxidative stress enzymes generally associated with anaerobic prokaryotes. Phylogenomic analysis identifies evidence for lateral gene transfer of bacterial genes into the E. histolytica genome, the effects of which centre on expanding aspects of E. histolytica's metabolic repertoire. The presence of these genes and the potential for novel metabolic pathways in E. histolytica may allow for the development of new chemotherapeutic agents. The genome encodes a large number of novel receptor kinases and contains expansions of a variety of gene families, including those associated with virulence. Additional genome features include an abundance of tandemly repeated transfer-RNA-containing arrays, which may have a structural function in the genome. Analysis of the genome provides new insights into the workings and genome evolution of a major human pathogen.