Plant growth-promoting rhizobacterial mediated protection in tomato against Tomato mottle virus
Plant growth-promoting rhizobacterial mediated protection in tomato against Tomato mottle virus
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DOI：
10.1094/pdis.2000.84.7.779
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发表时间：
2000-07-01
期刊：
影响因子：
4.5
通讯作者：
Kloepper, JW
中科院分区：
文献类型：
--
作者：
Murphy, JF;Zehnder, GW;Kloepper, JW
采用促生长根瘤菌（PGPR）作为工业配方种子处理，孢子制剂与盆栽培养基（称为粉末）混合，或种子-粉末联合处理，在田间条件下评估番茄植株对番茄斑疹病毒（ToMoV）的诱导抗性。根据其在先前实验中诱导抗性的能力，使用的PGPR菌株包括解淀粉芽孢杆菌937a、枯草芽孢杆菌937b和矮芽孢杆菌SE34。实验于1997年秋季和1998年春季和秋季在佛罗里达大学布雷登顿的墨西哥湾沿岸研究与教育中心进行。在移植后40天（数据），对所有植株的症状进行评分并分析ToMoV DNA的存在。在每个试验中，测定了单株上的粉虱密度，并在每个试验中至少测定了两次可销售的果实产量。最高水平的保护发生在1997年秋季的试验中，在40个数据时，所有PGPR粉末处理的ToMoV疾病严重程度评分明显低于种子或对照处理。利用Southern dot blot分析检测病毒DNA与症状严重程度等级相关，水果产量也是如此。在1998年春季的试验中，一些PGPR治疗也观察到ToMoV症状严重等级和病毒DNA发生率的降低，尽管相应的产量反应并不明显。在1998年秋季的试验中，几乎没有观察到耐药性。在80个数据的任何试验中，治疗之间在疾病严重程度、ToMoV DNA检测或产量方面没有差异。这些数据表明，在高水平载体病毒压力的自然条件下，多达40个数据，一些PGPR处理导致ToMoV发病率和疾病严重程度降低，在某些情况下，水果产量相应增加。使用PGPR可以成为管理番茄中这种病毒的综合计划的一个组成部分。
Tomato plants treated with plant growth-promoting rhizobacteria (PGPR), applied as an industrially formulated seed treatment, a spore preparation mixed with potting medium (referred to as powder), or a combined seed-powder treatment, were evaluated under field conditions for induced resistance to Tomato mottle virus (ToMoV). The PGPR strains used, based on their ability to induce resistance in previous experiments, included Bacillus amyloliquefaciens 937a, B. subtills 937b, and B. pumilus SE34. Experiments were conducted in the fall of 1997 and the spring and fall of 1998 at the University of Florida's Gulf Coast Research & Education Center, Bradenton. All plants were rated for symptoms and analyzed for the presence of ToMoV DNA at 40 days after transplant (dat). Whitefly densities were determined on individual plants in each trial, and marketable fruit yields were determined at least two times during each trial. The highest level of protection occurred in the fall 1997 trial when, at 40 dat, ToMoV disease severity ratings were significantly less in all PGPR powder-based treatments than in either of the seed or control treatments. Detection of viral DNA using Southern dot blot analyses correlated with symptom severity ratings, as did fruit yields. A reduction in ToMoV symptom severity ratings and incidence of viral DNA were also observed for some PGPR treatments in the spring 1998 trial, although corresponding yield responses were not apparent. Little or no resistance was observed in the fall 1998 trial. No differences in disease severity, detection of ToMoV DNA, or yield occurred among treatments in any of the trials at 80 dat. These data show that up to 40 dat under natural conditions of high levels of vector-virus pressure, some PGPR treatments resulted in reduced ToMoV incidence and disease severity and, in some cases, a corresponding increase in fruit yield. The use of PGPR could become a component of an integrated program for management of this virus in tomato.