FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat
FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat
复制标题
DOI：
10.1111/nph.17388
复制
发表时间：
2021-05-08
期刊：
影响因子：
9.4
通讯作者：
Ni, Zhongfu
中科院分区：
文献类型：
--
作者：
Du, Dejie;Zhang, Dongxue;Ni, Zhongfu
面包小麦（Triticum aestivum L.）很大程度上取决于花序结构。藏734是西藏特有的小麦品种，具有罕见的三小穗（TRS）表型，每穗小穗/小花数显著增加。通过图位克隆的方法，从藏734中分离到了TRS性状的致病基因。此外，利用基于CRISPR/Cas9的基因突变、转录组测序和蛋白质-蛋白质相互作用，确定了与小穗形成和芒伸长相关的下游信号网络。结果表明，WFZP-A的无效突变以及WFZP-D的缺失导致了Zang 734的TRS性状。更有趣的是，WFZP在同时抑制小穗形成基因TaBA 1和激活芒发育基因中起着双重作用，基本上通过招募染色质重塑元件和Mediator complex.我们的研究结果提供了WFZP抑制小穗形成但促进芒伸长的分子基础的见解，更重要的是，将WFZP-D定义为高产作物育种的有利基因。
Grain yield in bread wheat (Triticum aestivum L.) is largely determined by inflorescence architecture. Zang734 is an endemic Tibetan wheat variety that exhibits a rare triple spikelet (TRS) phenotype with significantly increased spikelet/floret number per spike. However, the molecular basis underlying this specific spike morphology is completely unknown.Through map-based cloning, the causal genes for TRS trait in Zang734 were isolated. Furthermore, using CRISPR/Cas9-based gene mutation, transcriptome sequencing and protein-protein interaction, the downstream signalling networks related to spikelet formation and awn elongation were defined.Results showed that the null mutation in WFZP-A together with deletion of WFZP-D led to the TRS trait in Zang734. More interestingly, WFZP plays a dual role in simultaneously repressing spikelet formation gene TaBA1 and activating awn development genes, basically through the recruitments of chromatin remodelling elements and the Mediator complex.Our findings provide insights into the molecular bases by which WFZP suppresses spikelet formation but promotes awn elongation and, more importantly, define WFZP-D as a favourable gene for high-yield crop breeding.