Chromosome analysis of single- and multipronucleated human zygotes proceeded after the intracytoplasmic sperm injection procedure
Chromosome analysis of single- and multipronucleated human zygotes proceeded after the intracytoplasmic sperm injection procedure
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胞浆内精子注射程序后对单核和多核人类受精卵进行染色体分析
DOI：
10.1007/bf02070150
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发表时间：
1996
影响因子：
3.1
通讯作者：
P. Keller
中科院分区：
文献类型：
--
作者：
E. Maćaš;B. Imthurn;Marinella Roselli;P. Keller
目的：通过将单个精子注射到细胞质中使卵子受精已被证明是人类体外受精中获得妊娠的有效方法。作为最具侵入性的显微操作技术之一，引起了人们对诱导胚胎异常的担忧。本研究的目的是深入了解胞浆内单精子注射程序后受精卵的染色体构成。方法：为此目的，在胞浆内单精子注射程序后对 33 个单核受精卵和 16 个多核受精卵进行细胞遗传学分析。结果：染色体扩散允许在 28 个（84.8%）单核受精卵中进行充分的核型分析。受精卵。其中，19 个（67.9%）为单倍体，5 个（17.9%）为亚单倍体（n=20-22），4 个（14.3%）为超单倍体（n=24-25）。这里发现的单核受精卵的非整倍体总体率为 32.1%。在分析的23个（82.1％）单原核受精卵中，除了单个有丝分裂补体外，还发现了完整的精子头或精子核结构，表明这些染色体的母体起源。 结论：注射单个精子后，鉴定出10个具有3个原核的双性受精卵和6个具有3个以上原核的受精卵，占所有受精卵母细胞的3.2％。在七个三核合子中的三个中发现了非整倍体染色体互补体，每个都表现出低、高和单倍体互补体（23，X，21，X，−A，−B，25，X，+A.+B；23，Y，22，X，−D，24，X，+D；23，X，22，X，−G，24，X，+G）。这两个不平衡的相应单倍型之间的染色体数目绝对差异是相同的，并且这种差异是由属于相同核型组的染色体引起的。在剩下的四个三核合子中，三个具有三倍体，一个具有二倍体染色体数。此外，在第一次分裂时具有三个以上原核的五个受精卵表现出严重耗尽的染色体互补体。大多数不平衡的多原核受精卵是在显微注射延迟后发现的，这表明卵母细胞的老化可能是其染色体排列异常的原因。
Purpose:Fertilization of an egg by injection of a single spermatozoon into the cytoplasm has been shown to be an effective procedure to obtain a pregnancy in human in vitro fertilization. This, as one of the most invasive micromanipulation techniques, has generated concern about inducing embryo abnormalities. The objective of this study was to obtain insight into the chromosomal constitution of zygotes proceeded after the intracytoplasmic sperm injection procedure.Methods:For this purpose the first cleavage division of 33 single- and 16 multipronucleated zygotes proceeded after the intracytoplasmic sperm injection procedure was cytogenetically analyzed.Results:Chromosome spreading permitted adequate karyotyping in 28 (84.8%) single-pronucleated zygotes. Among these, 19 (67.9%) were haploid, 5 (17.9%) hypohaploid (n=20–22), and 4 (14.3%) hyperhaploid (n=24–25). The overall rate of aneuploidy found here for single-pronucleated zygotes was 32.1%. In the 23 (82.1%) analyzed single-pronucleated zygotes, besides single mitotic complements, an intact sperm head or sperm nuclei structure has been found, indicating the maternal origin of these chromosomes.Conclusions:Ten digynic zygotes with three pronuclei and six zygotes with more than three pronuclei were identified after injecting a single spermatozoon, representing 3.2% of all the fertilized oocytes. Aneuploid chromosome complements were found in three of seven tripronuclear zygotes and each one exhibited a hypo-, hyper-, and haploid complement (23,X, 21,X,−A,−B, 25,X,+A.+B; 23,Y, 22,X,−D, 24,X,+D; 23,X, 22,X,−G, 24,X,+G). The absolute difference in the number of chromosomes between each of these two imbalanced corresponding haplotypes was the same and this difference was caused by the chromosomes belonging to the same groups of karyotype. Of the remaining four tripronuclear zygotes, three had triploid and one had diploid numbers of chromosomes. Furthermore, five zygotes having more than three pronuclei at the first cleavage division displayed severely depleted chromosome complements. The majority of imbalanced multipronuclear zygotes was found after delay of the microinjection, suggesting that aging of oocytes might be the reason for their abnormal chromosomal arrangements.