Use of high performance liquid chromatography-tandem mass spectrometry to distinguish Panax ginseng C. A. Meyer (Asian ginseng) and Panax quinquefolius L. (North American ginseng)
Use of high performance liquid chromatography-tandem mass spectrometry to distinguish Panax ginseng C. A. Meyer (Asian ginseng) and Panax quinquefolius L. (North American ginseng)
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DOI：
10.1021/ac000650l
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发表时间：
2000-11-01
影响因子：
7.4
通讯作者：
van Breemen, RB
中科院分区：
文献类型：
--
作者：
Li, WK;Gu, CG;van Breemen, RB
建立了液相色谱-串联质谱（LC-MS-RIS）鉴别亚洲人参（Panax ginseng C. A. Meyer）和北美人参（Panax quinquefolius L.）的方法。该方法基于人参根甲醇提取物中存在的两种潜在化学标志物人参皂苷Rf和24(R)-伪人参皂苷F-11的基线色谱分离，以及串联质谱法对其进行明确的在线鉴定。与文献一致，24(R)-伪人参皂苷F-11在北美人参根中的丰度超过干根的0.1% （w/w）。采用LC-RIS-MS法在亚洲人参根中也鉴定出了微量的24(R)-伪人参皂苷F-11，但含量小于0.0001% (w/w)，并与真品24(R)-伪人参皂苷F-11具有相同的串联质谱，在不同的HPLC条件下，用标准品洗脱了亚洲人参根中相应的化合物，从而确认该化合物为24(R)-伪人参皂苷F-11。另一种常用于区分亚洲人参和北美人参的人参皂苷，人参皂苷Rf，在亚洲人参根中的丰度大于0.021% （w/w）。在亚洲人参根中，人参皂苷Rf与24(R)-伪人参皂苷F-11的比值超过700:1。人参皂苷F-11或24(R)-伪人参皂苷F-11的柱上检出限为120 pg，柱上定量限为240 pg。综上所述，LC-MS-MS分析人参产品中人参皂苷Rf和24(R)-伪人参皂苷F-11的存在和比例可用于明确鉴别亚洲和北美人参。
A liquid chromatography-tandem mass spectrometry (LC-MS-RIS) method was developed to distinguish Asian ginseng (Panax ginseng C. A. Meyer) and North American ginseng (Panax quinquefolius L.). The method is based on the baseline chromatographic separation of ginsenoside Rf and 24(R)-pseudoginsenoside F-11, two potential chemical markers present in ginseng root methanolic extracts, and their unambiguous on-line identification using tandem mass spectrometry. Consistent with the literature, 24(R)-pseudoginsenoside F-11 was detected in abundance in North American ginseng roots in excess of 0.1% (w/w) of the dried root. In contrast to some reports, 24(R)-pseudoginsenoside F-11 was also identified in Asian ginseng roots at trace levels using LC-RIS-MS but at less than 0.0001% (w/w), Besides showing identical tandem mass spectra to authentic 24(R)-pseudoginsenoside F-11, the corresponding compound in Asian ginseng root coeluted with standard under different HPLC conditions, thus confirming this compound as 24(R)-pseudoginsenoside F-11. Another ginsenoside often used to distinguish Asian and North American ginseng, ginsenoside Rf, was found in abundance in Asian ginseng roots at more than 0.021% (w/w). In Asian ginseng roots, the ratio of ginsenoside Rf to 24(R)-pseudoginsenoside F-11 exceeded 700:1. The limit of detection of ginsenoside F-11 or 24(R)-pseudoginsenoside F-11 was 120 pg injected on-column, and the limit of quantification was 240 pg on-column. In summary, LC-MS-MS analysis of ginseng products for the presence and ratio of ginsenoside Rf and 24(R)-pseudoginsenoside F-11 may be used for the unambiguous identification of Asian and North American ginsengs.