Global transcriptional analysis of Streptococcus mutans sugar transporters using microarrays
Global transcriptional analysis of Streptococcus mutans sugar transporters using microarrays
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DOI：
10.1128/jb.00338-07
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Pham, Vi T. T.
中科院分区：
文献类型：
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作者：
Ajdic, Dragana;Pham, Vi T. T.
变形链球菌的碳水化合物转运是由磷酸烯醇丙酮酸-磷酸转移酶系统（PTS）和atp结合盒（ABC）转运体完成的。为了同时进行所有变形链球菌糖转运蛋白的全局转录分析，我们使用了全基因组表达微阵列。对几种单糖（葡萄糖、果糖、半乳糖和甘露糖）、双糖（蔗糖、乳糖、麦芽糖和海藻糖）、β -葡萄糖苷（纤维素二糖）、低聚糖（棉子糖、水苏糖和麦芽糖）和糖醇（甘露醇）测定了变形链球菌UA159的全局转录谱。结果表明，PTSs负责单糖、双糖、β -糖苷和糖醇的运输。只有在生长培养基中存在特定的糖时，6个PTSs才会被转录；因此，它们在转录水平上受到调控。其中包括果糖、乳糖、纤维素二糖和海藻糖的转运蛋白和甘露醇的两种转运蛋白。在所有测试条件下，三个PTSs均被抑制。有趣的是，无论使用的糖源如何，五种pts总是高度表达，这可能表明它们可以立即摄取最常见的膳食糖（葡萄糖、果糖、麦芽糖和蔗糖）。ABC转运蛋白被发现对低聚糖、棉子糖、水苏糖和异麦芽糖具有特异性。与PTSs相比，ABC转运蛋白在几种测试条件下表现出更高的转录水平，这表明它们可能运输多种底物。
The transport of carbohydrates by Streptococcus mutans is accomplished by the phosphoenolpyruvate-phosphotransferase system (PTS) and ATP-binding cassette (ABC) transporters. To undertake a global transcriptional analysis of all S. mutans sugar transporters simultaneously, we used a whole-genome expression microarray. Global transcription profiles of S. mutans UA159 were determined for several monosaccharides (glucose, fructose, galactose, and mannose), disaccharides (sucrose, lactose, maltose, and trehalose), a beta-glucoside (cellobiose), oligosaccharides (raffinose, stachyose, and maltotriose), and a sugar alcohol (mannitol). The results revealed that PTSs were responsible for transport of monosaccharides, disaccharides, beta-glucosides, and sugar alcohol. Six PTSs were transcribed only if a specific sugar was present in the growth medium; thus, they were regulated at the transcriptional level. These included transporters for fructose, lactose, cellobiose, and trehalose and two transporters for mannitoll. Three PTSs were repressed under all conditions tested. Interestingly, five PTSs were always highly expressed regardless of the sugar source used, presumably suggesting their availability for immediate uptake of most common dietary sugars (glucose, fructose, maltose, and sucrose). The ABC transporters were found to be specific for oligosaccharides, raffinose, stachyose, and isomaltosaccharides. Compared to the PTSs, the ABC transporters showed higher transcription under several tested conditions, suggesting that they might be transporting multiple substrates.