Two new fluorescent dyes applicable for visualization of fungal cell walls
Two new fluorescent dyes applicable for visualization of fungal cell walls
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DOI：
10.3852/mycologia.97.3.580
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发表时间：
2005-05-01
期刊：
影响因子：
2.8
通讯作者：
Turner, JN
中科院分区：
文献类型：
--
作者：
Hoch, HC;Galvani, CD;Turner, JN
报道了两种迄今未见报道的荧光团Solophenyl Flaine 7GFE 500和Pontamine Fast Scarlet 411，它们是显微镜下观察真菌细胞壁、隔膜和芽痕的有用染料。根据所使用的滤镜组件的不同，染料分别产生蓝色至绿色和黄色至红色波长的荧光染色物质，分别用于Solophenyl Flaine 7GFE 500和Pontamine坚固大红4B。它们提供了一个很好的替代更常用的荧光团，硫系氟白M2R。这两个荧光团除了在汞弧源的不同光谱波长上使用外，还可以与提供488 nm和543 mN线波长的激光源一起使用，这是大多数扫描共焦显微镜常见的。与硫氟剂不同的是，Solophenyl Flavine 7GFE 500和庞塔明坚固大红4B在选定的光波长下不会迅速褪色；然而，与硫氟剂一样，它们与活的真菌细胞也是相容的。
Two fluorophores, Solophenyl Flavine 7GFE 500 and Pontamine Fast Scarlet 411, not heretofore reported upon are described as useful dyes of fungal cell walls, septa and bud scars examined microscopically. The dyes, depending on the filter sets used, yield fluorescently stained material generally in the blue to green and yellow to red wavelengths for Solophenyl Flavine 7GFE 500 and Pontamine Fast Scarlet 4B, respectively. They provide an excellent alternative to the more commonly used fluorophore, Calcofluor White M2R. The two fluorophores, in addition to being used at various spectral wavelengths from mercury arc sources, can be used with laser sources providing 488 nm and 543 mn line wavelengths, common to most scanning confocal microscopes. Unlike Calcofluor, Solophenyl Flavine 7GFE 500 and Pontamine Fast Scarlet 4B do not fade quickly when exposed to selected light wavelengths; however, like Calcofluors they are compatible with living fungal cells.