HYALURONAN RECEPTOR (CD44) EXPRESSION AND FUNCTION IN HUMAN PERIPHERAL-BLOOD MONOCYTES AND ALVEOLAR MACROPHAGES
HYALURONAN RECEPTOR (CD44) EXPRESSION AND FUNCTION IN HUMAN PERIPHERAL-BLOOD MONOCYTES AND ALVEOLAR MACROPHAGES
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DOI：
10.1002/jlb.56.5.605
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发表时间：
1994-11-01
影响因子：
5.5
通讯作者：
SWARTZ, RP
中科院分区：
文献类型：
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作者：
CULTY, M;OMARA, TE;SWARTZ, RP
CD44 糖蛋白存在于许多造血细胞的表面，在某些情况下可以结合透明质酸（细胞外基质的主要成分）。在本研究中，我们发现新移植的人外周血单核细胞 (PBM) 表现出 85 kDa 的主要 CD44 带，而自体肺泡巨噬细胞 (AM phi) 表达范围从 85 到 200 kDa 的多种亚型。在培养 4 小时内，PBM 开始表达 120、150 和 180 kDa 的新 CD44 同工型。新移植的AM phi 特异性结合[H-3]透明质酸（135 cpm/mu g 蛋白质），但新移植的 PBM 则不然。然而，体外培养的PBM逐渐获得了结合[H-3]透明质酸的能力，并在培养4天后表现出与AM phi（113 cpm/μg蛋白）相似的透明质酸特异性结合。在这两种情况下，[H-3]乙酰透明质酸的结合均通过添加针对CD44的单克隆抗体而被特异性抑制。 AM phi 很容易降解[H-3]透明质酸，并在培养 4 天后达到稳定水平（115 cpm/mu g 蛋白质）。新移植的 PBM 没有表现出透明质酸降解，并且在培养 4 天后仅表现出很小的降解活性（6 至 11 cpm/mu g 蛋白质）。因此，随着 PBM 体外成熟，CD44 的表达和功能似乎发生变化，与 AM phi 中发现的更加相似。
CD44 glycoproteins are present on the surfaces of many hematopoietic cells and in some cases can bind hyaluronan, a major component of the extracellular matrix. In the present study, we have found that newly explanted human peripheral blood monocytes (PBMs) exhibit a major CD44 band of 85 kDa, whereas autologous alveolar macrophages (AM phi) express multiple isoforms ranging from 85 to 200 kDa. Within 4 h in culture, PBMs began expressing new CD44 isoforms of 120, 150, and 180 kDa. Newly explanted AM phi specifically bound [H-3]hyaluronan (135 cpm/mu g protein), but newly explanted PBMs did not. However, in vitro cultured PBM progressively acquired the ability to bind [H-3]hyaluronan and exhibited specific binding of hyaluronan similar to that of AM phi (113 cpm/mu g protein) after 4 days in culture. In both cases, the binding of [H-3]hyaluronan was specifically inhibited by the addition of monoclonal antibody directed against CD44. AM phi readily degraded [H-3]hyaluronan and reached a plateau after 4 days in culture (115 cpm/mu g protein). Newly explanted PBM exhibit no hyaluronan degradation and only a small deg radative activity after 4 days in culture (6 to 11 cpm/mu g protein). Thus, CD44 expression and function appear to change as PBM mature in vitro resembling more that found in AM phi.