EVIDENCE FOR A MULTIPLE INNERVATION OF PURKINJE-CELLS BY CLIMBING FIBERS IN IMMATURE RAT CEREBELLUM
EVIDENCE FOR A MULTIPLE INNERVATION OF PURKINJE-CELLS BY CLIMBING FIBERS IN IMMATURE RAT CEREBELLUM
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DOI：
10.1002/neu.480070609
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
DELHAYEBOUCHAUD, N
中科院分区：
文献类型：
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作者：
CREPEL, F;MARIANI, J;DELHAYEBOUCHAUD, N
在8至15日龄大鼠小脑上对爬行纤维（CF）-浦肯野细胞（PC）关系进行了电生理学研究。一些动物从出生起就通过X射线照射变得无颗粒;另一些动物在研究前3天用3-乙酰吡啶处理，以选择性破坏CF。8-9日龄正常大鼠的单一细胞外记录显示，超过50μ的PC单位各自表现出两种类型的全或无攀爬纤维反应（CFR）或逐步分级的CFR。其他PC装置仅显示一种全或无CFR。这些活性完全通过CF介导，因为它们在X射线照射的大鼠中持续相同的年龄，但在3-乙酰吡啶处理的动物中不存在。在8-9日龄正常大鼠49个PC单位上进行了顶旁区和下橄榄区刺激之间的相互作用实验。在24个只表现出一种类型的CFR的PC中，有21个在CF中建立的脉冲之间的碰撞被披露。在其他三台PC和显示两种全或无CFR或按步骤分级的CFR的25台PC中，均未检测到冲突。此外，在8-9日龄正常大鼠的PC中通过CFs介导的epsp的细胞内记录显示，它们经常以阶梯方式波动。这些结果表明，在未成熟大鼠中，超过50μ的PC中的每一个都受到至少两个不同的CF的神经支配;后来，CF和PC之间的额外突触的消失导致，早在第15天，CF和PC之间的一对一关系。
Climbing fiber (CF)–‐Purkinje cell (PC) relationships were studied electrophysiologically on the cerebellum of 8 to 15 day old rats. Some animals were rendered agranular by x‐irradiation from birth; some others were treated with 3‐acetyl pyridine 3 days before study to selectively destroy the CF. Unitary extracellular recordings in 8–9 day old normal rats revealed that more than 50μ of the PC units each exhibited either two types of all‐or‐none climbing fiber responses (CFR) or stepwise graded CFRs. The other PC units only presented one type of all‐or‐none CFR. These activities were entirely mediated via CF since they persisted at the same age in x‐irradiated rats, but were absent in animals treated with 3‐acetyl pyridine. Interaction experiments were performed between juxtafastigial and Inferior Olive stimulations on 49 PC units in 8–9 day old normal rats. Collisions between impulses set up in CFs were disclosed in 21 out of the 24 PCs which exhibited only one type of CFR. In the three others and in each of the 25 PCs that displayed two types of all‐or‐none CFRs, or CFRs graded by steps, no collision was detected. Moreover intracellular recordings of epsp's mediated via CFs in PCs of 8–9 day old normal rats revealed that they frequently fluctuated in stepwise fashion. These results demonstrate that in the immature rat more than 50μ of PCs are each innervated by at least two distinct CFs; later on, the disappearance of the supernumerary synapses between CF and PC leads, as early as day 15, to the one‐to‐one relationship between CF and PC.