DETECTION OF MYOSIN HEAVY-CHAIN MESSENGER-RNA DURING MYOGENESIS IN TISSUE-CULTURE BY INVITRO AND INSITU HYBRIDIZATION
DETECTION OF MYOSIN HEAVY-CHAIN MESSENGER-RNA DURING MYOGENESIS IN TISSUE-CULTURE BY INVITRO AND INSITU HYBRIDIZATION
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DOI：
10.1016/0092-8674(77)90126-x
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
JONES, KW
中科院分区：
文献类型：
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作者：
JOHN, HA;PATRINOUGEORGOULAS, M;JONES, KW
体外和原位杂交比较纯化鸡胚胎骨骼肌球蛋白H链mRNA （MHC mRNA）的c[互补]DNA拷贝，以区分肌源性和非肌源性细胞。在复制的单核细胞肌源性培养物中，大多数细胞不含可检测到的MHC mRNA。最早含有MHC mRNA的细胞是参与有丝分裂的细胞。在有丝分裂后的单核细胞和肌管中发现了相对大量的MHC mRNA，并且在这些细胞中观察到MHC mRNA的核仁定位。
A c[complementary]DNA copy of purified chick embryonic skeletal myosin H chain mRNA (MHC mRNA) distinguished between myogenic and nonmyogenic cells compared by in vitro and in situ hybridization. The majority of cells in replicating mononucleate myogenic cell cultures contained no detectable MHC mRNA. Among the earliest cells to contain MHC mRNA were cells engaged in mitosis. A relatively large amount of MHC mRNA was found in postmitotic monucleate cells and myotubes and nucleolar localization of MHC mRNA in these cells was observed.