CELL-MIGRATION AND SUBPLATE LOSS IN EXPLANT CULTURES OF MURINE CEREBRAL-CORTEX
CELL-MIGRATION AND SUBPLATE LOSS IN EXPLANT CULTURES OF MURINE CEREBRAL-CORTEX
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DOI：
10.1097/00001756-199307000-00018
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发表时间：
1993-07-01
期刊：
影响因子：
1.7
通讯作者：
PRICE, DJ
中科院分区：
文献类型：
--
作者：
GILLIES, K;PRICE, DJ
我们研究了在确定的无血清培养基中培养长达 14 天的小鼠皮质外植体中皮质细胞是否迁移和亚板细胞是否丢失。我们在胚胎第 12-13 天 (E12-13) 给小鼠注射溴脱氧尿苷 (BrdU)，以标记亚板细胞，或 E17，以标记前往浅层皮质层的细胞。我们从 E18-20 开始培养。我们留下一些小鼠来开发并提供体内数据。在 E18-20 时，大多数 E17 出生的细胞位于皮质板下方。在接下来的几天里，在体外，许多细胞移动到了皮质板的表面边缘，尽管异常多的细胞仍然留在心室区。培养物中的底板中 BrdU 标记的细胞选择性丢失；这比体内发生得更快。我们的培养物中缺乏的因素通常可能会增强亚板细胞的迁移并延长其存活时间。
WE studied whether cortical cells migrate and subplate cells are lost in explants of murine cortex cultured for up to 14 days in defined serum-free medium. We gave bromodeoxyuridine (BrdU) to mice on embryonic day 12-13 (E12-13), to label subplate cells, or E17, to label cells destined for superficial cortical layers. We started culturing on E18-20. We left some mice to develop and provide in vivo data. At E18-20, most cells born on E17 lay below the cortical plate. During the following days in vitro, many of them moved to the cortical plate's superficial edge, although an abnormally high number remained in the ventricular zone. There was a selective loss of BrdU labelled cells from the subplate in culture; this occurred more rapidly than in vivo. Factors absent from our cultures may normally enhance migration and prolong the survival of subplate cells.