Escherichia coli biofilm formation and recurrences of urinary tract infections in children
Escherichia coli biofilm formation and recurrences of urinary tract infections in children
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DOI：
10.1007/s10096-013-1935-4
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发表时间：
2014-01-01
影响因子：
4.5
通讯作者：
Uhari, M.
中科院分区：
文献类型：
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作者：
Tapiainen, T.;Hanni, A. -M.;Uhari, M.
有研究表明，尿路致病性大肠杆菌（UPEC）分离物形成的生物膜与尿路感染（UTI）的复发和持续有关。我们比较了急性和复发性UTI儿童UPEC分离株的体外生物膜形成。采用206例连续临床UPEC分离物，这些分离物来自证实为UTI的儿童，即肾盂肾炎（n = 78）、复发性肾盂肾炎（n = 10）、膀胱炎（n = 84）或复发性膀胱炎（n = 34），我们在聚苯乙烯微滴板上应用1%结晶紫染色，在72 h后测量光密度（OD）值。该方法在共聚焦激光扫描显微镜和扫描电子显微镜下进行了生物膜形成测量。复发性膀胱炎组的OD值低于其他组（平均OD值0.36，SD值0.21 vs平均OD值0.47，SD值0.36,P = 0.04），而复发性肾盂肾炎组的OD值高于其他组（平均OD值0.69，SD值0.33 vs平均OD值0.44，SD值0.34,P = 0.006），提示引起复发性肾盂肾炎的菌株形成生物膜。似乎upc分离物在非生物表面有效生长生物膜所需的特性对复发性肾盂肾炎很重要，但对复发性膀胱炎不重要。分析体外观察到的大肠杆菌的生物膜特性是否预示着抗菌药物治疗的临床反应较慢，以及尿路感染后肾瘢痕形成的增加，这在未来是有价值的。
It has been suggested that biofilm formation by uropathogenic Escherichia coli (UPEC) isolates is associated with recurrence and persistence of urinary tract infection (UTI). We compared the in vitro biofilm formation of UPEC isolates from children with acute or recurrent UTI. Employing 206 consecutive clinical UPEC isolates from children with proven UTI, i.e., pyelonephritis (n = 78), recurrent pyelonephritis (n = 10), cystitis (n = 84) or recurrent cystitis (n = 34), we applied 1 % crystal violet staining to polystyrene microtitre plates at 72 h and measured the optical density (OD) values. The method had been validated to measure biofilm formation against confocal laser scanning microscopy and scanning electron microscopy. The OD values were lower in the recurrent cystitis group than in the other groups (mean OD 0.36, SD 0.21 vs mean 0.47, SD 0.36, P = 0.04) and higher in the recurrent pyelonephritis group than in the other groups (mean OD 0.69, SD 0.33 vs mean OD 0.44, SD 0.34, P = 0.006) indicating biofilm formation of strains causing recurrent pyelonephritis. It appears that the properties of UPEC isolates required for effective biofilm growth on an abiotic surface are important for recurrent pyelonephritis, but not for recurrent cystitis. It would be valuable in the future to analyze whether the biofilm properties of E. coli observed in vitro predict a slower clinical response to antimicrobial treatment and increased renal scar formation after UTI.