[Photodynamic diagnosis and preoperative chemotherapy for biliary tract cancer].
[Photodynamic diagnosis and preoperative chemotherapy for biliary tract cancer].
复制标题
胆道癌的光动力诊断及术前化疗[J].
DOI：
--
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
G. Wakabayashi
中科院分区：
文献类型：
--
作者：
Naoko Ito;A. Sugitachi;Masahiro Takahashi;Kenji Makabe;S. Kanno;Yasushi Hasegawa;T. Takahara;T. Fujita;S. Nishizuka;H. Nitta;G. Wakabayashi
背景
癌细胞从氨基乙酰丙酸(ALA)合成大量的原卟啉IX(PPIX)。PPIX在蓝光下照射时会发出红色荧光。基于这一现象的光动力诊断(PDD)目前正在使用；然而，各种微生物在蓝光下也显示出与ALA相同的荧光，导致PDD结果假阳性。
目的和方法
为了避免误诊，我们在PDD系统中加入了新的系统。ALA、蓝光(波长380-450 nm)、不同种类的细胞株和细菌被用于体外研究。以自行研制的70%脱乙酰化壳聚糖溶液(DAC-70Sol)为抗菌剂，制备了新型光显像剂AlA/DAC-70Sol。对ALA/DAC-70溶胶的体外抗菌性能进行了检测，并用胆道癌患者的胆汁进行了临床光诊断实验。
结果
DAC-70溶胶具有良好的体外杀菌作用。可以清楚地识别红色荧光，从而能够使用ALA/DAC-70 Sol检测胆汁中的癌细胞。
结论
我们的新系统在临床光动力细胞诊断(PDCD)中有很大的应用潜力，这在癌症术前化疗中发挥着重要作用。
BACKGROUND
Cancer cells synthesize substantial amounts of protoporphyrin IX( PPIX) from aminolevulinic acid( ALA). PPIX emits red fluorescence when illuminated under blue light. Photodynamic diagnosis (PDD), based on this phenomenon, is currently used; however, various microorganisms also show the same fluorescence with ALA when illuminated under blue light, resulting in false-positive PDD results.
PURPOSE AND METHODS
To avoid misdiagnosis, we incorporated novel systems into the PDD system. ALA, blue light (wavelength, 380-450 nm), different kinds of cell lines, and bacteria were used in this in vitro study. We used a 70% deacetylated chitosan solution (DAC-70 Sol), developed in-house, as an antibacterial agent and prepared ALA/DAC-70 Sol, used as a novel photoimaging agent. The antibacterial function of ALA/DAC-70 Sol was examined in vitro, and the photodiagnostic effects on using the novel systems were clinically evaluated using bile from patients with biliary tract cancer.
RESULTS
DAC-70 Sol demonstrated an effective bactericidal function in vitro. Red fluorescence could clearly be identified, enabling the detection of cancer cells in the bile using ALA/DAC-70 Sol.
CONCLUSIONS
Our novel systems have a great potential for use in clinical photodynamic cytodiagnosis( PDCD), which plays an important role in preoperative cancer chemotherapy.