Using engineered endonucleases to create knockout and knockin zebrafish models.
Using engineered endonucleases to create knockout and knockin zebrafish models.
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DOI：
10.1007/978-1-4939-1862-1_17
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Ekker, Stephen C
中科院分区：
文献类型：
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作者：
Bedell, Victoria M;Ekker, Stephen C
在过去的几年里，创造有针对性的敲除和敲入斑马鱼动物的技术已经爆炸。我们已经获得了通过使用锌指核酸酶（ZFN）、转录激活因子样效应物核酸酶（TALEN）和成簇的规则间隔短回文重复序列/CRISPR相关系统（CRISPR/Cas）产生靶向敲除的能力。此外，使用高效的TALEN系统，我们能够使用这里描述的单链DNA（ssDNA）协议创建敲入斑马鱼。通过这些技术的应用，斑马鱼已经成为一种有价值的脊椎动物模型，也是研究人类疾病的无脊椎动物和哺乳动物模型系统之间的极好桥梁。
Over the last few years, the technology to create targeted knockout and knockin zebrafish animals has exploded. We have gained the ability to create targeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create knockin zebrafish using a single-stranded DNA (ssDNA) protocol described here. Through the use of these technologies, the zebrafish has become a valuable vertebrate model and an excellent bridge between the invertebrate and mammalian model systems for the study of human disease.