RAPID PROTEIN EXTRACTION FROM SACCHAROMYCES-CEREVISIAE
RAPID PROTEIN EXTRACTION FROM SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/yea.320101007
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发表时间：
1994-10-01
期刊：
影响因子：
2.6
通讯作者：
RIEZMAN, H
中科院分区：
文献类型：
--
作者：
HORVATH, A;RIEZMAN, H
我们已经开发和评估了一种简单，快速的方法从酵母细胞中提取蛋白质的十二烷基硫酸钠（SDS）-凝胶电泳和蛋白质印迹。该程序包括离心步骤以收获细胞，加入样品缓冲液并加热，然后在将上清液中发现的提取的蛋白质应用于SDS凝胶之前进行另一个离心步骤。适用于1天内大量样品的研究。该方法与使用碱和2-巯基乙醇的方法一样简单、快速和有效，但在某些条件下比使用玻璃珠的裂解效率略低。
We have developed and evaluated an easy and rapid method for extraction of proteins from yeast cells for sodium dodecyl sulphate (SDS)-gel electrophoresis and Western blotting. The procedure comprises a centrifugation step to harvest the cells, addition of a sample buffer and heating, then another centrifugation step before applying the extracted proteins found in the supernatant to an SDS gel. It is applicable to the study of large numbers of samples in 1 day. This procedure is easier, quicker, and as efficient as procedures using base and 2-mercaptoethanol, but somewhat less efficient than lysis with glass beads under certain conditions.