Down-regulate of Djrfc2 causes tissues hypertrophy during planarian regeneration
Down-regulate of Djrfc2 causes tissues hypertrophy during planarian regeneration
复制标题
Djrfc2 下调导致涡虫再生过程中组织肥大
DOI：
10.1016/j.bbrc.2017.09.032
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发表时间：
2017-11-25
影响因子：
3.1
通讯作者：
Zhang, Shoutao
中科院分区：
文献类型：
--
作者：
Guo, Qi;Zhao, Guixia;Zhang, Shoutao
涡虫具有惊人的再生能力，是再生研究的理想模式生物。DNA复制对基因组的稳定性至关重要。复制因子C （Replication factor C， RFC）是一种复制因子C样复合体，在真核生物DNA复制过程中起重要作用，已被报道为涡虫再生过程中的伤口反应因子。然而，RFC如何通过调节DNA复制来控制涡虫的再生仍有待解释。在这里，我们使用二维电泳（2-DE）蛋白质组学方法来鉴定完整和再生涡虫的差异表达蛋白。在完整组织和再生组织之间，大约有132个蛋白点显示出差异。我们选择了21个显著表达的蛋白点，并对其进行了TOF MS分析。最后，我们克隆了三个候选基因（Djhsp70, Djrfc2, Djfaim），重点研究了Djrfc2在再生过程中的功能。我们发现Djrfc2的分布倾向于伤口部位。Djrfc2的RNA干扰（RNAi）增加了分裂细胞的数量和涡虫新生细胞标记基因的表达水平，可能导致超增殖。我们的研究采用了一种可行的方法，直接研究了蛋白质水平上的再生动态，并为支持Djrfc2在涡虫再生中的作用提供了进一步的证据。(C) 2017年Elsevier Inc.出版。
Planarians are an ideal model organism for regeneration research due to their amazing ability to regenerate. DNA replication is crucial for genome stability. Replication factor C (RFC), which is a replication factor C-like complex and plays an important role during DNA replication in eukaryotes, has been reported as a wound response factor during planarian regeneration. However, how RFC controls regeneration in planarians by regulating DNA replication remains to be explained. Here, we used a twodimensional electrophoresis (2-DE) proteomic approach to identify differentially expressed proteins in intact and regenerated planarians. Approximately 132 protein spots showed differences between intact and regenerative tissues. We selected 21 significantly expressed protein spots and processed them using TOF MS analysis. Finally, we cloned three of these candidate genes (Djhsp70, Djrfc2, Djfaim), focusing on the function of Djrfc2 during regeneration. We found that the distribution of Djrfc2 tends toward the wound site. RNA interference (RNAi) of Djrfc2 increases the number of dividing cells and the expression level of planarian neoblast marker genes, which may result in hyper-proliferation. Our studies use an available approach to directly study the regeneration dynamic at the protein level and provide further evidence to support a function of Djrfc2 in planarian regeneration. (C) 2017 Published by Elsevier Inc.