Long Dwell-Time Exposure of Human Chorionic Villi to Transvaginal Ultrasound in the First Trimester of Pregnancy Induces Activation of Caspase-3 and Cytochrome C Release
Long Dwell-Time Exposure of Human Chorionic Villi to Transvaginal Ultrasound in the First Trimester of Pregnancy Induces Activation of Caspase-3 and Cytochrome C Release
复制标题
妊娠早期经阴道超声长时间暴露于人绒毛膜可诱导 Caspase-3 激活和细胞色素 C 释放
作者：
Jiayin Zhang;F. Zhou;Yiling Song;W. Ying;Ying Zhang
摘要 暴露于超声波后的生物效应与其持续时间相关。尽管诊断超声被认为可以诱导细胞凋亡，但潜在的信号转导途径仍然难以捉摸。在这项研究中，妊娠前三个月的女性接受频率为 5.0 MHz 的经阴道诊断超声波 0、10、20 或 30 分钟。暴露后 4 小时获得绒毛膜绒毛，并通过蛋白质印迹分析 caspase-3 的激活和细胞色素 c 的释放。与0分钟和10分钟组相比，20分钟和30分钟组中活性caspase-3和细胞色素c释放的裂解产物显着增加，且呈时间依赖性。我们发现，在妊娠前三个月，长时间暴露于经阴道超声会激活效应器 caspase-3 介导的绒毛膜绒毛凋亡级联反应。这是通过参与细胞色素 c 释放的内在死亡途径发生的。我们的研究结果为妊娠早期区分使用经阴道超声提供了分子原理。
Abstract Bioeffects after exposure to ultrasound are correlated to its duration. Although diagnostic ultrasound has been suggested to induce apoptosis, the underlying signal transduction pathway remains elusive. In this study, women in the first trimester of pregnancy were exposed to transvaginal diagnostic ultrasound with 5.0-MHz frequency for 0, 10, 20, or 30 min. The chorionic villi were obtained 4 h after exposure and activation of caspase-3 and cytochrome c release were analyzed by Western blotting. In contrast with the 0- and 10-min groups, cleavage products of active caspase-3 and cytochrome c release significantly increased in 20- and 30-min groups in a time-dependent manner. We show that long-duration exposure to transvaginal ultrasound activates effector caspase-3-mediated apoptotic cascade of chorionic villi in the first trimester of pregnancy. This occurs through the intrinsic death pathway involved in cytochrome c release. Our findings provide a molecular rationale for discriminant use of transvaginal ultrasound at the early stage of pregnancy.
p53 依赖性 DNA 损伤诱导的细胞凋亡需要 Fas/APO-1 独立的 CPP32beta 激活。
影响因子：
11.2
作者：
Fuchs,EJ;McKenna,KA;Bedi,A
通讯作者：
Bedi,A