Phytosterols in sea buckthorn (Hippophae rhamnoides L.) berries:: Identification and effects of different origins and harvesting times
Phytosterols in sea buckthorn (Hippophae rhamnoides L.) berries:: Identification and effects of different origins and harvesting times
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DOI：
10.1021/jf010813m
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发表时间：
2001-11-01
影响因子：
6.1
通讯作者：
Kallio, HP
中科院分区：
文献类型：
--
作者：
Yang, B;Karlsson, RM;Kallio, HP
沙棘（Hippophae rhamnoides L.）来自芬兰和中国的属于两个主要亚种（sinensis和rhamnoides）的样品在油皂化后通过气相色谱-质谱法分析为TMS衍生物。种子、果肉/果皮和整个浆果中的总甾醇含量分别为1200-1800、240-400和340-520 mg/kg。提取油中的相应值为12-23、10-29和13-33 g/kg。谷甾醇分别占种子和果肉/果皮甾醇的57- 76%和61- 83%。甾醇含量和组成表明亚种和采集地点之间的变化不大。不同采收期对种子和果肉/果皮中甾醇类化合物的含量有显著影响。所获得的甾醇谱可用于表征沙棘和检测有价值的油的掺假。本研究为进一步研究沙棘甾醇的化学性质和开发利用沙棘作为功能性食品原料提供了重要依据。
Sterols in seeds, pulp/peel fractions, and whole berries of sea buckthorn (Hippophae rhamnoides L.) samples belonging to two major subspecies (sinensis and rhamnoides) from Finland and China were analyzed as TMS derivatives by gas chromatography-mass spectrometry after saponification of the oils. The total sterol contents, in the seeds, the fresh pulp/peel, and the whole berries were, 1200-1800, 240-400, and 340-520 mg/kg, respectively. The corresponding values in the extracted oils were 12-23, 10-29, and 13-33 g/kg. Sitosterol constituted 57-76 and 61-83%, respectively, of the seed and pulp/peel sterols. The sterol content and composition showed little variation between subspecies and collection sites. Different harvesting dates showed significant effects on the levels of some sterols both in the seeds and in the pulp/peel. The sterol profiles obtained are useful for characterizing sea buckthorn and detecting adulterations of the valuable oils. The information provided by the present investigation is also important for further chemical investigation of sea buckthorn sterols and industrial utilization of the berries as a raw material of functional foods.