LEGIONNAIRES DISEASE - ISOLATION OF A BACTERIUM AND DEMONSTRATION OF ITS ROLE IN OTHER RESPIRATORY-DISEASE
LEGIONNAIRES DISEASE - ISOLATION OF A BACTERIUM AND DEMONSTRATION OF ITS ROLE IN OTHER RESPIRATORY-DISEASE
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DOI：
10.1056/nejm197712012972202
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发表时间：
1977-01-01
影响因子：
158.5
通讯作者：
DOWDLE, WR
中科院分区：
文献类型：
--
作者：
MCDADE, JE;SHEPARD, CC;DOWDLE, WR
为了确定退伍军人病的病原体，对患者的血清和组织样本进行了检查，以寻找毒素、细菌、真菌、衣原体、立克次体和病毒。从 6 名患者中 4 名的豚鼠肺部分离出革兰氏阴性、非抗酸杆菌。芽孢杆菌可以转移到含胚蛋的卵黄囊中。该生物体的分类不完整。芽孢杆菌的卵黄囊培养物被用作抗原，使用抗人球蛋白荧光抗体来调查可疑的血清样本。与对照相比，111 名符合退伍军人病临床标准的患者中有 101 名的样本显示出抗体滴度的诊断性增加。最近 54 例散发的严重肺炎病例以及其他 2 起先前未解决的呼吸道疾病暴发中大多数患者的储存血清中也发现了诊断性增加。军团病显然是由革兰氏阴性细菌引起的，这种细菌可能导致广泛的感染。
To identify the etiologic agent of Legionnaires'' disease, patients'' serum and tissue specimens were examined in a search for toxins, bacteria, fungi, chlamydiae, rickettsiae and viruses. From the lungs of 4 of 6 patients a gram-negative, non-acid-fast bacillus was isolated in guinea pigs. The bacillus could be transferred to yolk sacs of embryonated [chicken] eggs. Classification of this organism is incomplete. Yolk-sac cultures of the bacillus were used as antigen to survey suspected serum specimens, employing antihuman-globulin fluorescent antibody. When compared to controls, specimens from 101 of 111 patients meeting clinical criteria of Legionnaires'' disease showed diagnostic increases in antibody titers. Diagnostic increases were also found in 54 recent sporadic cases of severe pneumonia and, retrospectively, in stored serum from most patients in 2 other previously unsolved outbreaks of respiratory disease. Legionnaires'' disease is apparently caused by a gram-negative bacterium that may be responsible for widespread infection.