Cloning and endonuclease mapping of the hepatitis B viral genome
Cloning and endonuclease mapping of the hepatitis B viral genome
复制标题
乙型肝炎病毒基因组的克隆和核酸内切酶作图
作者：
J. Sninsky;A. Siddiqui;W. Robinson;S. Cohen
引起乙型肝炎或血清肝炎的病毒在自然界中似乎只感染人类，并且仅在少数其他哺乳动物中实现了实验性感染。乙型肝炎病毒 (HBV) 的宿主范围有限，而且迄今为止未能感染组织培养细胞，这极大地限制了对该病毒的研究，并阻碍了针对其引起的严重疾病的疫苗的开发。我们在这里报告了在大肠杆菌 K12 中克隆双链 HBV DNA，利用病毒基因组上独特的 EcoRI 切割位点将整个 HBV DNA 分子引入 pACYC184 质粒载体的氯霉素 (Cm) 抗性基因内的 EcoRI 切割位点。
THE virus that causes hepatitis B, or serum hepatitis, seems to infect only humans in nature, and experimental infection has been achieved in only a few additional mammals. The limited host range of the hepatitis B virus (HBV), and its failure so far to infect tissue culture cells have drastically restricted study of this virus and have hindered development of a vaccine for the serious disease that it causes. We report here the cloning of double-stranded HBV DNA in Escherichia coli K12, using the unique EcoRI cleavage site on the viral genome to introduce the entire HBV DNA molecule into an EcoRI cleavage site within the chloroamphenicol (Cm) resistance gene of the pACYC184 plasmid vector1.