β-Hydroxybutyric sodium salt inhibition of growth hormone and prolactin secretion via the cAMP/PKA/CREB and AMPK signaling pathways in dairy cow anterior pituitary cells.
β-Hydroxybutyric sodium salt inhibition of growth hormone and prolactin secretion via the cAMP/PKA/CREB and AMPK signaling pathways in dairy cow anterior pituitary cells.
复制标题
DOI：
10.3390/ijms16024265
复制
发表时间：
2015-02-16
影响因子：
5.6
通讯作者：
Wang JF
中科院分区：
文献类型：
--
作者：
Fu SP;Wang W;Liu BR;Yang HM;Ji H;Yang ZQ;Guo B;Liu JX;Wang JF
β-羟基丁酸（BHBA）调节生长激素（GH）和催乳素（PRL）的合成和分泌，但其机制尚不清楚。在本研究中，我们检测了BHBA对奶牛垂体前叶细胞（DCAPC）中G蛋白信号通路活性、AMPK-α活性、GH和PRL基因转录以及GH和PRL分泌的影响。结果表明，BHBA 降低了细胞内 cAMP 水平，并随后降低了蛋白激酶 A (PKA) 活性。抑制 PKA 活性可降低 cAMP 反应元件结合蛋白 (CREB) 磷酸化，从而抑制 GH 和 PRL 转录和分泌。 BHBA 的作用被一种特定的 Gαi 抑制剂百日咳毒素 (PTX) 减弱。此外，与高糖组相比，在低糖和无糖条件下培养的DCAPCs中，单羧酸转运蛋白1（MCT1）介导的细胞内BHBA摄取可触发AMPK信号传导，导致GH和PRL mRNA翻译减少。本研究确定了BHBA对DCAPCs中GH和PRL基因转录、翻译和分泌的调节作用的生化机制，这可能是奶牛围产期垂体功能的调节因素之一。
β-hydroxybutyric acid (BHBA) regulates the synthesis and secretion of growth hormone (GH) and prolactin (PRL), but its mechanism is unknown. In this study, we detected the effects of BHBA on the activities of G protein signaling pathways, AMPK-α activity, GH, and PRL gene transcription, and GH and PRL secretion in dairy cow anterior pituitary cells (DCAPCs). The results showed that BHBA decreased intracellular cAMP levels and a subsequent reduction in protein kinase A (PKA) activity. Inhibition of PKA activity reduced cAMP response element-binding protein (CREB) phosphorylation, thereby inhibiting GH and PRL transcription and secretion. The effects of BHBA were attenuated by a specific Gαi inhibitor, pertussis toxin (PTX). In addition, intracellular BHBA uptake mediated by monocarboxylate transporter 1 (MCT1) could trigger AMPK signaling and result in the decrease in GH and PRL mRNA translation in DCAPCs cultured under low-glucose and non-glucose condition when compared with the high-glucose group. This study identifies a biochemical mechanism for the regulatory action of BHBA on GH and PRL gene transcription, translation, and secretion in DCAPCs, which may be one of the factors that regulate pituitary function during the transition period in dairy cows.
登录
查看更多内容
影响因子：
5.8
作者：
Chai, Joshua T.;Digby, Janet E.;Choudhury, Robin P.
通讯作者：
Choudhury, Robin P.
影响因子：
32.4
作者：
Singh N;Gurav A;Sivaprakasam S;Brady E;Padia R;Shi H;Thangaraju M;Prasad PD;Manicassamy S;Munn DH;Lee JR;Offermanns S;Ganapathy V
通讯作者：
Ganapathy V
DOI：
10.1016/j.jsbmb.2014.02.009
发表时间：
2014-09-01
影响因子：
4.1
作者：
Li, Xinwei;Li, Yu;Li, Xiaobing
通讯作者：
Li, Xiaobing
影响因子：
64.8
作者：
DOWDEN, DR;JACOBSON, DR
通讯作者：
JACOBSON, DR
影响因子：
3.5
作者：
Laporta, J.;Driver, A.;Khatib, H.
通讯作者：
Khatib, H.