Genetic variant of SPARC gene and its association with growth traits in Chinese cattle
Genetic variant of SPARC gene and its association with growth traits in Chinese cattle
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DOI：
10.5194/aab-63-31-2020
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发表时间：
2020-01
影响因子：
1.8
通讯作者：
D. Zhang;Jiawei Xu;Peng Yang;Yifan Wen;Hua He;Jiaxiao Li;Juntong Liang;Yining Zheng;
中科院分区：
文献类型：
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作者：
D. Zhang;Jiawei Xu;Peng Yang;Yifan Wen;Hua He;Jiaxiao Li;Juntong Liang;Yining Zheng;
摘要β-半胱氨酸是一种富含半胱氨酸的酸性分泌蛋白。它是骨的非胶原成分，广泛分布于人和动物体内，起着重要的作用。在多种人类癌症（乳腺癌、胃癌、卵巢癌等）中发现了癌基因。和糖尿病相关的研究。尤其是肌肉和脂肪的代谢密切相关。在这项研究中，我们使用一个DNA池来检测一个新的SNP位点（g.12454T> C）。采用ARMS-PCR技术对秦川牛（QC，n = 176）、夏南牛（XN，n = 160）、皮南牛（PN，n = 136）和佳县牛（JX，n = 144）4个品种共616份样品进行了分析和鉴定。此外，我们还将SNP与生长性状进行了相关分析，结果表明SNP与猪的臀长、臀宽、体长等生长性状相关显著（p <0.05）。此外，我们测试了XN成年牛（n = 3）不同组织中的表达水平，发现其在肌肉组织中的高表达（相对于肾脏）。此外，我们发现SNP能够增加骨骼肌中的β-淀粉样蛋白表达水平（n = 12）。根据统计数据，该SNP位点可应用于肉牛早期生长的早期标记辅助选择的分子标记。
Abstract SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T > C). A total of 616 samples of four breeds of Qinchuan cattle (QC, n=176), Xianan cattle (XN, n=160), Pinan cattle (PN, n=136) and Jiaxian cattle (JX, n=144) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length (p<0.05). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle (n=3) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle (n=12). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle.