Development of non-viral vectors for systemic gene delivery
Development of non-viral vectors for systemic gene delivery
复制标题
DOI：
10.1016/s0168-3659(01)00494-1
复制
发表时间：
2002-01-17
影响因子：
10.8
通讯作者：
Huang, L
中科院分区：
文献类型：
--
作者：
Liu, F;Huang, L
基因治疗的主要挑战之一是系统地将核酸直接输送到受影响的组织中，这需要开发一种能够保护核酸不被降解的载体，同时将感兴趣的基因输送到特定的组织和特定的亚细胞室。在这篇综述中，我们总结了用于全身给药的新的非病毒递送系统的一些最新进展。描述了两种类型的基因递送系统：(I)LPD1(阳离子脂质体包裹，聚阳离子缩合DNA，类型1)，和(Ii)保留时间介导的裸露DNA递送。对这些系统性基因转移的假设机制也进行了讨论。(C)2002 Elsevier Science B.V.保留所有权利。
dOne of the major challenges for gene therapy is systemic delivery of a nucleic acid directly into an affected tissue, This requires developing a vehicle which is able to protect the nucleic acid from degradation, while delivering the gene of interest to the specific tissue and specific subcellular compartment. In this review, we summarize some of the recent advances in new non-viral delivery systems for systemic administration. Two types of gene delivery systems are described: (i) LPD1 (cationic liposome-entrapped, polycation-condensed DNA, type 1), and (ii) retention-time mediated naked DNA delivery. Hypothesized mechanisms for these systemic gene transfers are also discussed. (C) 2002 Elsevier Science B.V. All rights reserved.