Characterization of microrough bioactive glass surface:: Surface reactions and osteoblast responses in vitro
Characterization of microrough bioactive glass surface:: Surface reactions and osteoblast responses in vitro
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DOI：
10.1002/jbm.10273
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发表时间：
2002-12-05
期刊：
影响因子：
--
通讯作者：
Aro, HT
中科院分区：
文献类型：
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作者：
Itälä, A;Ylänen, HO;Aro, HT
目前的研究特点的微粗糙的生物活性玻璃的体外表面反应，并比较光滑和微粗糙表面之间的成骨细胞的反应。三种不同的生物活性玻璃组合物被使用，并使用一种新的化学蚀刻方法获得表面微观粗糙化。将烧结微球制成的多孔生物活性玻璃试样浸入模拟体液（SBF）或三羟甲基氨基甲烷（Tris）溶液中1、6、24、48或72 h，通过扫描电子显微镜/能量色散X射线分析（SEM/EDXA）研究反应层的形成。在生物活性玻璃盘上进行细胞培养研究，以检查表面微粗糙度对人成骨样细胞（MG-63）的附着和增殖的影响。通过原位染色细胞的显微镜计数来评价细胞附着。采用非放射性细胞增殖试验结合原位染色和激光共聚焦显微镜分析细胞增殖。生物活性玻璃表面的微粗糙化增加了在浸泡的第一个小时期间硅胶层形成的速率。磷酸钙层的形成在控制和微粗糙化玻璃表面之间是相等的。在生物活性玻璃上的细胞培养中，与光滑玻璃表面相比，微粗糙表面增强了成骨样细胞的附着，但对细胞的增殖速率或形态没有影响。总之，微粗糙化显著加速了三种生物活性玻璃表面反应的早期形成，并对初始细胞附着产生了积极影响。(C)2002 Wiley Periodicals，Inc.
The current study characterized the in vitro surface reactions of microroughened bioactive glasses and compared osteoblast cell responses between smooth and micro-rough surfaces. Three different bioactive glass compositions were used and surface microroughening was obtained using a novel chemical etching method. Porous bioactive glass specimens made of sintered microspheres were immersed in simulated body fluid (SBF) or Tris solutions for 1, 6, 24, 48, or 72 h, and the formation of reaction layers was studied by means of a scanning electron microscope/energy dispersive X-ray analysis (SEM/EDXA). Cell culture studies were performed on bioactive glass disks to examine the influence of surface microroughness on the attachment and proliferation of human osteoblast-like cells (MG-63). Cell attachment was evaluated by means of microscopic counting of in situ stained cells. Cell proliferation was analyzed with a nonradioactive cell proliferation assay combined with in situ staining and laser confocal microscopy. The microroughening of the bioactive glass surface increased the rate of the silica gel layer formation during the first hours of the immersion. The formation of calcium phosphate layer was equal between control and microroughened glass surfaces. In cell cultures on bioactive glass, the microrough surface enhanced the attachment of osteoblast-like cells but did not have an effect on the proliferation rate or morphology of the cells as compared with smooth glass surface. In conclusion, microroughening significantly accelerated the early formation of surface reactions on three bioactive glasses and had a positive effect on initial cell attachment. (C) 2002 Wiley Periodicals, Inc.