Proliferation and cell shape changes during ciliary body morphogenesis in the mouse
Proliferation and cell shape changes during ciliary body morphogenesis in the mouse
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DOI：
10.1002/dvdy.20302
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发表时间：
2005-05-01
影响因子：
2.5
通讯作者：
Kidson, SH
中科院分区：
文献类型：
--
作者：
Napier, HRL;Kidson, SH
关于小鼠眼中睫状突的结构和发育知之甚少。我们的扫描电子显微镜（SEM）研究表明，与其他哺乳动物不同，纤毛突形成一种不规则的图案，交叉和交织，而不是彼此平行。从胚胎 (E) 14.5 天到出生后 (P) 7 天的组织学和扫描电镜研究表明，睫状区的第一个形态学标志是环形隆起；然后逐渐将其模制成离散的纤毛突起。发育中的毛细血管网络和成人睫状皱襞之间的惊人相似性表明，纤毛过程的图案模板可能是潜在的毛细血管网络。细胞增殖测量和细胞高度评估表明，在纤毛过程的形态发生过程中发生的首要事件之一是外睫状上皮中 PO 周围的增殖激增。这种激增和细胞高度的增加很可能导致该层的膨胀。稍微延迟后，内部睫状上皮通过增殖并向晶状体向内延伸来做出反应。睫状突的最终成形是通过内部睫状上皮细胞高度的降低来实现的。因此，在小鼠中，睫状体形态发生过程中有丝分裂和细胞高度变化之间的时间相关性表明这些过程在睫状过程的形成中发挥着不可或缺的作用。 (c) 2005 年 Wiley-Liss, Inc.
Very little is known about the structure and development of the ciliary processes in the mouse eye. Our scanning electron microscope (SEM) studies reveal that, unlike other mammals, the ciliary processes form an irregular pattern, crossing over and interweaving rather than lying parallel to one another. Histological and SEM studies from embryonic day (E) 14.5 to postnatal day (P) 7 reveal that the first morphological sign of the ciliary zone is an annular bulge; this is then gradually molded to form discrete ciliary processes. The striking similarity between the developing capillary network and the adult ciliary folds suggests that the patterning template for the ciliary processes could be the underlying capillary network. Cell proliferation measurements and cell height assessments indicated that one of the first events occurring during the morphogenesis of ciliary processes is a proliferative surge around PO in the outer ciliary epithelium. It is likely that this surge together with increasing cell heights leads to a bulging of this layer. After a slight delay, the inner ciliary epithelium responds by proliferating and extending inward toward the lens. Final shaping of the ciliary processes is achieved through cell height reductions in the inner ciliary epithelium. Thus, in the mouse, the temporal correlation between mitotic and cell height changes during ciliary body morphogenesis suggests that these processes play an integral role in the shaping of ciliary processes. (c) 2005 Wiley-Liss, Inc.