Intracranial pressure measured in freely moving rats for days after intracerebral hemorrhage
Intracranial pressure measured in freely moving rats for days after intracerebral hemorrhage
复制标题
DOI：
10.1016/j.expneurol.2014.02.017
复制
发表时间：
2014-05-01
影响因子：
5.3
通讯作者：
Colbourne, Frederick
中科院分区：
文献类型：
--
作者：
Hiploylee, Carmen;Colbourne, Frederick
在一些患者中，由于血肿和水肿的团块效应，脑出血（ICH）会导致危及生命的颅内压（ICP）升高。因此，水肿是衡量啮齿动物脑出血模型治疗效果的常见终点。尽管广泛依赖水肿，但其与ICP和脑灌注压（CPP）的关系尚不清楚。在纹状体注射胶原酶（“重度”- 0.3 U，“中度”- 0.15 U）或血液（100 μ L）（与生理盐水注射对照）后，用遥测装置测量大鼠血压（BP）和ICP。我们比较了硬膜外和实质内的ICP读数（胶原酶），评估了CPP（胶原酶），并比较了模型。中度（9.46 mm Hg +/- 4.72 SD， 3天平均）和重度胶原酶ICHs（10.79 +/- 3.50）与假手术相比显著增加ICP(4.02 +/- 2.09)，而输血没有（5.37 +/- 0.55）。在严重的胶原酶脑出血后，两个监测位置的读数相似。在大量胶原酶输注后数天内，ICP升高使CPP降低7.5 mm Hg。各组平均CPP为103-112毫米汞柱。所有脑出血模型均出现水肿，可预测颅内压。然而，即使在严重脑出血和水肿后，ICP和CPP也只有轻微的变化。因此，文献中通常报道的水肿的微小变化，通常使用比目前使用的更小的出血，可能对ICP和CPP的影响最小。需要进一步研究这些模型的表面有效性、终点及其评估治疗方法的能力。(C) 2014年作者。Elsevier Inc.出版。
In some patients, intracerebral hemorrhage (ICH) causes life-threatening elevations in intracranial pressure (ICP) arising from mass effect of the hematoma and edema. Accordingly, edema is a common endpoint to gauge treatment efficacy in rodent ICH models. Despite widespread reliance on edema, its relationship with ICP and cerebral perfusion pressure (CPP) is unknown. Blood pressure (BP) and ICP were measured by telemetry devices in rats after collagenase ("severe" - 0.3 U, and "moderate" - 0.15 U doses) or blood infusion (100 mu L) into striatum (vs. saline infused shams). We compared epidural and intraparenchymal ICP readings (collagenase), evaluated CPP (collagenase), and compared models. Moderate (9.46 mm Hg +/- 4.72 SD, 3 day average) and severe collagenase ICHs (10.79 +/- 3.50) significantly increased ICP versus shams (4.02 +/- 2.09), whereas blood infusion did not (5.37 +/- 0.55). The two monitoring locations gave similar readings after severe collagenase ICH. Increased ICP reduced CPP by similar to 7.5 mm Hg for days after the larger collagenase infusion. CPP averaged from 103-112 mm Hg in shams. Edema occurred in all ICH models and predicted ICP. However, ICP and CPP were only modestly changed even after severe ICH and edema. Thus, small changes in edema typically reported in the literature, which often use smaller bleeds than presently used, likely minimally affects ICP and CPP. Further research into the face validity of these models, endpoints, and their ability to evaluate therapeutics is needed. (C) 2014 The Authors. Published by Elsevier Inc.