A novel, thermostable manganese-containing superoxide dismutase from Bacillus licheniformis
A novel, thermostable manganese-containing superoxide dismutase from Bacillus licheniformis
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DOI：
10.1007/s10529-010-0368-8
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发表时间：
2010-12-01
影响因子：
2.7
通讯作者：
Emanuilova, Elka
中科院分区：
文献类型：
--
作者：
Boyadzhieva, Ivanka Petrova;Atanasova, Miroslava;Emanuilova, Elka
一种新的，热稳定的超氧化物歧化酶（SOD）从地衣芽孢杆菌M20，从保加利亚矿泉分离，纯化11倍，11%的活性回收率。从非变性PAGE和SDS-PAGE分析，该酶由两个亚基组成，每个亚基21.5kDa。SOD仅被NaN_3抑制，表明该SOD为锰超氧化物歧化酶型。纯化的酶在pH 8和55 ℃时具有最大活性。95 ℃时SOD的半衰期为10 min。
A new, thermostable superoxide dismutase (SOD) from Bacillus licheniformis M20, isolated from Bulgarian mineral springs, was purified 11-fold with 11% recovery of activity. From native PAGE and SDS-PAGE, the enzyme was composed of two subunits of 21.5 kDa each. The SOD was inhibited only by NaN3, which suggested that this SOD is of the manganese superoxide dismutase type. The purified enzyme had maximum activity at pH 8 and 55 degrees C. The half-life of the SOD was 10 min at 95 degrees C.