Interaction between hydrogenase, nitrogenase, and respiratory activities in a Frankia isolate from Alnus rubra.
Interaction between hydrogenase, nitrogenase, and respiratory activities in a Frankia isolate from Alnus rubra.
复制标题
从赤杨中分离出的 Frankia 中氢化酶、固氮酶和呼吸活动之间的相互作用。
DOI：
10.1139/m89-102
复制
发表时间：
1989
影响因子：
2.8
通讯作者：
M. F. López
中科院分区：
文献类型：
--
作者：
M. Murry;M. F. López
测定了从赤杨根瘤中分离的Frankia ArI 3菌株的固氮培养物的吸H2和吸H2支持的O2。完整细胞的H2摄取是O2依赖性的，在环境O2浓度下观察到最大速率。没有氢酶活性可以检测到NH4+生长，未分化的细丝有氧培养，表明摄取氢酶活性与囊泡，细胞的固氮在Frankia网站。乙炔对氢化酶活性有抑制作用，但用H2预处理可减轻抑制作用。H2刺激乙炔还原在超最佳，但不是次优的O2浓度。这些结果表明，摄取氢酶活性ArI 3可能发挥作用，在固氮酶的O2保护，特别是在碳限制的条件下。
H2 uptake and H2-supported O2 uptake were measured in N2-fixing cultures of Frankia strain ArI3 isolated from root nodules of Alnus rubra. H2 uptake by intact cells was O2 dependent and maximum rates were observed at ambient O2 concentrations. No hydrogenase activity could be detected in NH4+-grown, undifferentiated filaments cultured aerobically indicating that uptake hydrogenase activity was associated with the vesicles, the cellular site of nitrogen fixation in Frankia. Hydrogenase activity was inhibited by acetylene but inhibition could be alleviated by pretreatment with H2. H2 stimulated acetylene reduction at supraoptimal but not suboptimal O2 concentrations. These results suggest that uptake hydrogenase activity in ArI3 may play a role in O2 protection of nitrogenase, especially under conditions of carbon limitation.