AN 18-MONTH STUDY OF THE EFFECTS ON HAMSTER LUNGS OF INTRATRACHEALLY ADMINISTERED HUMAN NEUTROPHIL ELASTASE
AN 18-MONTH STUDY OF THE EFFECTS ON HAMSTER LUNGS OF INTRATRACHEALLY ADMINISTERED HUMAN NEUTROPHIL ELASTASE
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DOI：
10.3109/01902148809087836
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发表时间：
1988-01-01
影响因子：
1.7
通讯作者：
SNIDER, GL
中科院分区：
文献类型：
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作者：
LUCEY, EC;STONE, PJ;SNIDER, GL
研究了单次气管内注射350 .mu的雄性叙利亚金仓鼠肺内肺气肿和气道损伤的演变过程。g人中性粒细胞弹性蛋白酶（HNE）。分别于治疗后1、3、6、12、18个月对生理盐水对照组和hne治疗组8只动物进行研究。在所有研究时间内，HNE治疗导致所有肺容量显著增加，最大呼气流量显著减少。左肺平均线性截距（MLI）值较对照组显著升高。MLI值、肺体积或肺顺应性没有随时间进展。分泌细胞化生在1个月时出现，并在整个研究过程中持续存在。经hne处理的肺在终末空气中可见含铁巨噬细胞团簇。肺铁含量在1个月时最高，6个月时下降，18个月时没有变化。18个月时，铁含量仍显著高于对照组。我们得出结论，HNE引起的气道和实质病变持续18个月无进展。铁的清除可能是HNE治疗引起的出血的结果，持续了6个月，没有明显的后续清除。
A study was made of the evolution of emphysema and airway injury induced in the lungs of male golden Syrian hamsters by a single intratracheal injection of 350 .mu.g human neutrophil elastase (HNE). Saline control and HNE-treated groups of 8 animals were studied 1,3,6,12 and 18 months posttreatment. HNE treatment caused a significant increase in all lung volumes and a significant decrease in maximum expiratory flows at all study times. The mean linear intercept (MLI) values of the left lung were significantly increased over control values. There was no progression with time in MLI values, lung volumes, or lung compliance. Secretory-cell metaplasia was present at 1 month and persisted throughout the study. The HNE-treated lungs showed clusters of ferric iron-containing macrophages in the terninal airspaces. The amount of iron in the lungs, determined morphometrically, was greatest at 1 month, was decreased by 6 months, and then did not change further to 18 months. At 18 months the amount of iron was still significantly above control amounts. We conclude that the airway and parenchymal lesions induced by HNE persist without progression for 18 months. Clearance of ferric iron, which was probably a result of the hemorrhage induced by HNE treatment, continued for 6 months with no evident subsequent clearance.