Rabbit Retinal Neovascularization Induced by Latex Angiogenic-Derived Fraction: An Experimental Model
Rabbit Retinal Neovascularization Induced by Latex Angiogenic-Derived Fraction: An Experimental Model
复制标题
乳胶血管生成组分诱导兔视网膜新生血管形成：实验模型
作者：
R. B. Sampaio;R. Mendonça;A. R. Simioni;R. Costa;R. Siqueira;V. M. Corrêa;A. Tedesco;A. Haddad;J. Coutinho Netto;R. Jorge
目的：玻璃体腔注射乳胶衍生血管生成组分微球建立视网膜新生血管实验模型。方法：32只白化病新西兰兔，分为4组，每组8只。第I、II和III组的家兔右眼分别接受一次玻璃体内注射PLGA（L-丙交酯-共-乙交酯）微球（含10、30和50 µg乳胶衍生的血管生成组分），第IV组接受0.1 ml不含血管生成组分的微球。每周进行眼底镜检查和荧光素血管造影随访;在第4周处死兔，并对它们的眼睛进行光学显微镜检查。结果如下：组I的所有眼睛均表现出视网膜血管迂曲度增加，从注射后14天开始观察并维持28天，否则未检测到新血管。所有II组眼睛显示与I组相似的血管变化。注射后21天，第II组兔的50%的眼睛发生视网膜新生血管形成。III组的所有眼睛在注射后2周表现出显著的血管迂曲和视网膜新生血管，进展为纤维血管增生和牵拉性视网膜脱离。IV组无血管改变或视网膜新生血管。光学显微镜检查证实，在邻近视盘的视网膜切片中，存在先前在荧光素血管造影中观察到的新血管，而在对照组的相同区域的切片中未观察到。结论：将含有乳胶衍生的血管生成组分的30 μ g和50 μg微球注射到兔的玻璃体腔中诱导视网膜新生血管形成。
Purpose: To create a retinal neovascularization experimental model using intravitreal injection of microspheres loaded with latex-derived angiogenic fraction. Methods: Thirty-two albino New Zealand rabbits, divided in 4 groups of 8 animals, were enrolled in this study. Rabbits in groups I, II, and III received one intravitreal injection of PLGA (L-lactide-co-glycolide) microspheres with 10, 30, and 50 µg of latex-derived angiogenic fraction into their right eyes, respectively, and group IV received 0.1 ml of microspheres without the angiogenic fraction. Weekly follow-up with ophthalmoscopy and fluorescein angiography was performed; the rabbits were sacrificed in the 4th week and their eyes processed for light microscopy. Results: All eyes from group I demonstrated increased retinal vascular tortuosity, observed from 14 days after injection and maintained for 28 days, otherwise without new vessels detection. All group II eyes showed vascular changes similar to group I. Fifty percent of the eyes from group II rabbits developed retinal neovascularization 21 days after injection. All eyes from group III demonstrated significant vascular tortuosity and retinal new vessels 2 weeks after injection, progressing to fibrovascular proliferation and tractional retinal detachment. No vascular changes or retinal new vessels were observed in group IV eyes. Light microscopy confirmed the existence of new vessels previously seen on fluorescein angiography, in retinal sections adjacent to the optic disc, not observed in sections at the same area in the control group. Conclusion: Thirty- and 50-µg microspheres containing latex-derived angiogenic fraction injected into the vitreous cavity induced retinal neovascularization in rabbits.
影响因子：
4.4
作者：
Lois E. H. Smith;Eva Wesoloiuski;Angela McLellan;Sandra K. Kostyk;Robert D'Amato;Richard Sullivan
通讯作者：
Lois E. H. Smith;Eva Wesoloiuski;Angela McLellan;Sandra K. Kostyk;Robert D'Amato;Richard Sullivan
影响因子：
3.4
作者：
H. Ozaki;H. Hayashi;S. Vinores;Y. Moromizato;P. Campochiaro;K. Oshima
通讯作者：
H. Ozaki;H. Hayashi;S. Vinores;Y. Moromizato;P. Campochiaro;K. Oshima
影响因子：
13.7
作者：
R. Klein;B. Klein;S. Moss
通讯作者：
R. Klein;B. Klein;S. Moss