Synaptic integration of olfactory information in mouse anterior olfactory nucleus
Synaptic integration of olfactory information in mouse anterior olfactory nucleus
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DOI：
10.1523/jneurosci.2598-06.2006
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发表时间：
2006-11-15
影响因子：
5.3
通讯作者：
Katz, Lawrence C.
中科院分区：
文献类型：
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作者：
Lei, Huimeng;Mooney, Richard;Katz, Lawrence C.
个别气味激活只有一小部分的二尖瓣细胞在小鼠的主要嗅球（MOB）。气味混合物由激活的僧帽细胞的组合表示，在嗅球中形成可再现的激活图。然而，如何激活一个队列的窄调谐的二尖瓣细胞的气味混合物是由神经元在更高层次的嗅觉结构，如前嗅核（AON）的突触读出，大多是未知的。在目前的研究中，我们使用细胞内和细胞外的记录，检查和比较AON神经元和MOB二尖瓣细胞的反应面板的结构不同的气味剂呈现为混合物或单独的组件。我们发现，大多数单个AON神经元可以被几种结构不同的成分的混合物和几种不同的成分在一个有效的混合物中突触激活。AON神经元对有效混合物的阈上响应通常超过其对该混合物中所有组分的阈上响应的总和，表明非线性组合相互作用。与AON神经元的广泛反应相反，大多数二尖瓣细胞仅被单一混合物中的一种或两种组分激活。AON神经元相对于二尖瓣细胞的更广泛的反应性表明，单个AON神经元突触整合几个功能不同的二尖瓣细胞输入。
Individual odorants activate only a small fraction of mitral cells in the mouse main olfactory bulb ( MOB). Odor mixtures are represented by a combination of activated mitral cells, forming reproducible activation maps in the olfactory bulb. However, how the activation of a cohort of narrowly tuned mitral cells by odor mixtures is read out synaptically by neurons in higher-level olfactory structures, such as the anterior olfactory nucleus (AON), is mostly unknown. In the current study, we used intracellular and extracellular recordings to examine and compare responses of AON neurons and MOB mitral cells to a panel of structurally diverse odorants presented either as mixtures or as individual components. We found that a majority of individual AON neurons could be synaptically activated by several mixtures of structurally dissimilar components and by several dissimilar components in an effective mixture. The suprathreshold response of an AON neuron to an effective mixture often exceeded the sum of its suprathreshold responses to all of the components in that mixture, indicating a nonlinear combinatorial interaction. In contrast to the broad responsiveness of AON neurons, the majority of mitral cells were activated by only one or two components in a single mixture. The broader responsiveness of AON neurons relative to mitral cells suggests that individual AON neurons synaptically integrate several functionally distinct mitral cell inputs.