Clinical significance of enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid
Clinical significance of enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid
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DOI：
10.1016/s0923-1811(00)00149-3
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发表时间：
2001-05-01
影响因子：
4.6
通讯作者：
Hashimoto, T
中科院分区：
文献类型：
--
作者：
Amo, Y;Ohkawa, T;Hashimoto, T
180 kDa 大疱性类天疱疮抗原 (BP180) 的 NC16A 结构域是大疱性类天疱疮 (BP) 中最具免疫原性且可能致病的区域。在本研究中，为了确定循环抗 BP180 自身抗体的血清水平是否是 BP 中有价值的血清标志物，使用酶联免疫吸附测定 (ELISA) 测量了 10 名 BP 患者血清针对 BP180 NC16A 结构域的免疫反应性。接受各种治疗药物的 BP 患者的血清抗 BP180 自身抗体水平与临床病程相关。结果表明，NC16A-ELISA 是评估 BP 患者临床病程和治疗效果的有用方法。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
The NC16A domain of the 180-kDa bullous pemphigoid antigen (BP180) is the most immunogenic and, probably, pathogenic region in bullous pemphigoid (BP). In the present study, in order to determine whether serum level of circulating anti-BP180 autoantibodies is a valuable serum marker in BP, the immunoreactivity of sera against the NC16A domain of BP180 was measured using enzyme-linked immunosorbent assay (ELISA) in ten patients with BP. Serum levels of anti-BP180 autoantibodies correlated with the clinical course in BP patients, who received various therapeutic agents. The result suggests that this NC16A-ELISA is a useful method for evaluating the clinical course and efficacy of the therapy in patients with BP. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.