Analysis of microbial components using LC-IR.
Analysis of microbial components using LC-IR.
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DOI：
10.1021/ac034571w
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发表时间：
2003-07
影响因子：
7.4
通讯作者：
S. Huffman;Kara B. Lukasiewicz;S. Geldart;S. Elliott;J. Sperry;Chris W. Brown
中科院分区：
文献类型：
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作者：
S. Huffman;Kara B. Lukasiewicz;S. Geldart;S. Elliott;J. Sperry;Chris W. Brown
细菌的表征是当前医学、军事、食品和农业科学中的重要研究领域。近年来，傅立叶变换红外光谱已被用作微生物检测方法和一般研究工具。当与液相色谱系统相结合时，研究的一个新方面已经发展起来。通过利用典型的液相色谱系统的分离能力，基质消除是可能的，因此允许细胞组分的清洁光谱。关于各种细菌组成的信息增强了对细胞水平生物学的整体理解，提供了细胞过程化学的量化，并可用作一般识别工具。在本研究中，对全细胞和裂解的大肠杆菌细胞进行了研究。通过FT-IR分离、分离和鉴定了由蛋白质、糖蛋白、磷脂、脂肪酰胺和脂肪酸组成的细胞组分以及基因组物质。
Characterization of bacteria is currently an important research area in the medical, military, food, and agricultural sciences. In recent years, FT-IR has found an application as a microbiological detection method and as a general research tool. When coupled with a liquid chromatographic system, a new facet of research has evolved. By utilizing the separation ability of typical liquid chromatography systems, matrix elimination is possible, therefore allowing for clean spectra of cellular components. Information about the compositional makeup of various bacteria enhances the overall understanding of biology at the cellular level, provides a quantification of the chemistry of cellular processes, and can be used as a general identification tool. Both whole cells and lysed Escherichia coli cells were investigated in the present study. The cellular components consisting of proteins, glycoproteins, phospholipids, fatty amides and acids, and genomic materials were separated, isolated, and identified by FT-IR.