Plant hormone effects on isoprene emission from tropical tree in Ficus septica
Plant hormone effects on isoprene emission from tropical tree in Ficus septica
复制标题
植物激素对热带榕树异戊二烯排放的影响
DOI：
10.1111/pce.13513
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Oku Hirosuke
中科院分区：
文献类型：
--
作者：
Parveen Shahanaz;Iqbal Md. Asif;Mutanda Ishmael;Rashid Md. Harun‐Ur‐;Inafuku Masashi;Oku Hirosuke
植物激素和昼夜节律参与了异戊二烯排放的协调控制。为了深入了解信号网络，植物激素的叶面施用在天然发射器中进行，Ficus septica。喷施50 μM茉莉酸（JA）后，异戊二烯排放量在5d内逐渐降低，降低幅度达88%，缓解后排放量增加。通过分析光合速率、2-C-甲基-D-赤藓醇4-磷酸（MEP）途径基因表达、激素信号传导和昼夜节律过程以及MEP途径代谢物库大小，进一步探讨了异戊二烯释放的分子调控机制。结果表明，茉莉酸处理期间异戊二烯的释放与异戊二烯合成酶（IspS）基因表达和IspS蛋白水平密切相关，表明茉莉酸对IspS的转录和翻译调控。JA协同调控基因在生长素、细胞分裂素和昼夜节律信号转导途径中的应用。在所分析的转录因子中，MYC 2（JA）和LHY（生物钟）与异戊二烯排放呈负相关。IspS启动子上预测的推定元件（MYC 2的G盒和LHY的昼夜节律）支持我们的提议，即异戊二烯的释放是通过植物激素和昼夜节律信号对IspS基因的协调转录调节来调节的。
Plant hormones and the circadian rhythm have been implicated in coordinated control of isoprene emission in plants. To gain insights into the signalling networks, foliar application of plant hormones was conducted in a native emitter,Ficus septica. Spraying of 50 μM jasmonic acid (JA) gradually decreased isoprene emission by 88% compared with initial levels within 5 days, and emission increased after relief from JA application. We further explored the molecular regulatory mechanism of isoprene emission by analysing photosynthetic rate, gene expression of 2‐C‐methyl‐D‐erythrytol 4‐phosphate (MEP) pathway, hormone signalling and circadian rhythm processes, and metabolite pool sizes of MEP pathway. Results show that isoprene emission strongly correlated with isoprene synthase (IspS) gene expression and IspS protein levels over the period of JA treatment, indicating transcriptional and possible translational modulation ofIspSby JA. Application of JA coordinately modulated genes in the auxin, cytokinin (CK), and circadian rhythm signal transduction pathways. Among the transcriptional factors analysed,MYC2(JA) andLHY(circadian clock) negatively correlated with isoprene emission. Putativecis‐elements predicted onIspSpromoter (G‐box forMYC2and circadian forLHY) supports our proposal that isoprene emission is regulated by coordinated transcriptional modulation ofIspSgene by phytohormone and circadian rhythm signalling.