Effects of negative pressures on epithelial tight junctions and migration in wound healing
Effects of negative pressures on epithelial tight junctions and migration in wound healing
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DOI：
10.1152/ajpcell.00504.2009
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发表时间：
2010-08-01
影响因子：
5.5
通讯作者：
Wang, Jong-Shyan
中科院分区：
文献类型：
--
作者：
Hsu, Chih-Chin;Tsai, Wen-Chung;Wang, Jong-Shyan
徐冲，蔡武昌，陈正平，陆永明，王建生。负压对伤口愈合中上皮紧密连接和迁移的影响。Am J Physiol Cell Physiol 299：C528-C534,2010。2010年5月5日首次出版；DOI：10.1152/ajpcell.00504.2009。-负压伤口疗法最近在慢性伤口护理中越来越受欢迎。本研究试图探讨不同负压对伤口愈合过程中上皮细胞迁移的影响。采用细胞-底物阻抗传感(ECIS)技术，在狗肾(MDCK)和人角质形成细胞(HaCaT)细胞上制作了5×10(-4)cm(2)的创面。损伤细胞在负压培养箱中培养，环境压力为75 mm Hg(NP75)，负压为125 mm Hg(NP125)，负压为175 mm Hg(NP175)。观察不同压力下有效时间(ET)、创面完全愈合时间(T-max)、愈合率(R-Hear)、细胞直径和创面面积随时间的变化。传统的伤口愈合试验用于细胞活力、细胞连接蛋白(包括ZO-1和E-钙粘蛋白)和肌动蛋白的荧光染色。AP和NP125处的细胞连接蛋白的数量也进行了定量。在MDCK细胞中，NP125的ET(1.25+/-0.27 h)、T-max(1.76+/-0.32 h)和R-Hear(2.94+/-0.62×10(-4)cm(2)/h)与其他三种压力条件下的ET(1.25±-0.27 h)、T-max(1.76+/-0.32 h)和R-Hear(2.94+/-0.62×10(-4)cm(2)/h)有显著差异(P<；0.01)。在HaCaT细胞中，NP125的Tmax(7.34+/-0.29h)和R-Hear(6.82+/-0.26×10(-5)cm(2)/h)与NP75的T-max(7.34+/-0.29h)和R-Hear(6.82+/-0.26×10(-5)cm(2)/h)有显著差异(P<；0.01)。在特定负压下，细胞有明显的迁移特征。不同压力下的细胞迁移活动可以用实时伤口愈合测量系统记录下来。负压125毫米汞柱可以帮助分解细胞连接，促进上皮迁移，随后导致伤口快速闭合。
Hsu CC, Tsai WC, Chen CP, Lu YM, Wang JS. Effects of negative pressures on epithelial tight junctions and migration in wound healing. Am J Physiol Cell Physiol 299: C528-C534, 2010. First published May 5, 2010; doi: 10.1152/ajpcell.00504.2009.-Negative-pressure wound therapy has recently gained popularity in chronic wound care. This study attempted to explore effects of different negative pressures on epithelial migration in the wound-healing process. The electric cell-substrate impedance sensing (ECIS) technique was used to create a 5 x 10(-4) cm(2) wound in the Madin-Darby canine kidney (MDCK) and human keratinocyte (HaCaT) cells. The wounded cells were cultured in a negative pressure incubator at ambient pressure (AP) and negative pressures of 75 mmHg (NP75), 125 mmHg (NP125), and 175 mmHg (NP175). The effective time (ET), complete wound healing time (T-max), healing rate (R-heal), cell diameter, and wound area over time at different pressures were evaluated. Traditional wound-healing assays were prepared for fluorescent staining of cells viability, cell junction proteins, including ZO-1 and E-cadherin, and actins. Amount of cell junction proteins at AP and NP125 was also quantified. In MDCK cells, the ET (1.25 +/- 0.27 h), T-max (1.76 +/- 0.32 h), and R-heal (2.94 +/- 0.62 x 10(-4) cm(2)/h) at NP125 were significantly (P < 0.01) different from those at three other pressure conditions. In HaCaT cells, the T-max (7.34 +/- 0.29 h) and R-heal (6.82 +/- 0.26 x 10(-5) cm(2)/h) at NP125 were significantly (P < 0.01) different from those at NP75. Prominent cell migration features were identified in cells at the specific negative pressure. Cell migration activities at different pressures can be documented with the real-time wound-healing measurement system. Negative pressure of 125 mmHg can help disassemble the cell junction to enhance epithelial migration and subsequently result in quick wound closure.