The histone variant CENP-A and centromere specification
The histone variant CENP-A and centromere specification
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DOI：
10.1016/j.ceb.2007.11.007
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发表时间：
2008-02-01
影响因子：
7.5
通讯作者：
Bassett, Emily A.
中科院分区：
文献类型：
--
作者：
Black, Ben E.;Bassett, Emily A.
着丝粒是染色体的基因座，指导忠实的遗传。着丝粒是表观遗传学上指定的，组蛋白H3变体CENP-A已成为携带表观遗传学着丝粒标记的最佳候选者。最近的进展证明了这种表观遗传标记的物理基础，CENP-A赋予它与其他核心组蛋白形成的核小体构象刚性。这种核小体被组成型着丝粒蛋白的多亚基复合物识别，称为CENP-A（NAC）。来自不同真核生物中的两个CENP-A亲属的证据表明，它们形成的组蛋白复合物在DNA上采用了高度非常规的排列。着丝粒身份本身在有丝分裂退出和早期G1期间传播，并且它依赖于CENP-A内的顺式作用靶向结构域和提出的着丝粒“引发”反应。
The centromere is the chromosomal locus that guides faithful inheritance. Centromeres are specified epigenetically, and the histone H3 variant CENP-A has emerged as the best candidate to carry the epigenetic centromere mark. Recent advances demonstrate the physical basis for this epigenetic mark whereby CENP-A confers conformational rigidity to the nucleosome it forms with other core histones. This nucleosome is recognized by a multisubunit complex of constitutive centromere proteins, termed the CENP-A(NAC). Evidence from two CENP-A relatives in diverse eukaryotes suggests that the histone complexes they form adopt highly unconventional arrangements on DNA. Centromere identity, itself, is propagated during mitotic exit and early G1, and it relies upon a cis-acting targeting domain within CENP-A and a proposed centromere 'priming' reaction.