Influence of Diffusion on Excitation Energy Transfer in Solutions by Gigahertz Harmonic Content Frequency-Domain Fiuorometry.
Influence of Diffusion on Excitation Energy Transfer in Solutions by Gigahertz Harmonic Content Frequency-Domain Fiuorometry.
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DOI：
10.1021/j100385a011
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发表时间：
1990-11
期刊：
影响因子：
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通讯作者：
J. Lakowicz;H. Szmacinski;I. Gryczynski;W. Wiczk;Michaell . Johnson
中科院分区：
文献类型：
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作者：
J. Lakowicz;H. Szmacinski;I. Gryczynski;W. Wiczk;Michaell . Johnson
我们报告了扩散对荧光共振能量转移的影响，正如使用频域技术观察到的那样。研究了吲哚（供体）和丹西酰胺（受体）在低粘度和高粘度溶液中的能量转移。在目前的分辨率水平下，根据Gosele等人提出的包括平移扩散在内的理论函数，对供体荧光衰减进行了令人满意的分析。理论物理。左。1975,24,519)。研究发现，不考虑扩散的福斯特方程明显高估了流体溶液中的临界传递距离（r0），并且不能充分表示数据。这些结果证明了从供体发射的频率响应确定相互扩散系数的能力。
We report the effects of diffusion on fluorescence resonance energy transfer, as observed by using the frequency-domain technique. Energy transfer between indole (donor) and dansylamide (acceptor) was studied in low and high viscosity solutions. At the current level of resolution, the donor fluorescence decays were satisfactorily analyzed in terms of the theoretical function including translational diffusion proposed by Gosele et al. (Chem. Phys. Lett. 1975, 24, 519). The Forster equation, which does not consider diffusion, was found to significantly overestimate the critical transfer distance (R 0) in fluid solutions and to provide an inadequate representation of the data. These results demonstrate the ability to determine mutual diffusion coefficients from the frequency response of the donor emission.