A specific and biocompatible fluorescent sensor based on the hybrid of GFP chromophore and peptide for HSA detection
A specific and biocompatible fluorescent sensor based on the hybrid of GFP chromophore and peptide for HSA detection
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一种基于 GFP 发色团和肽混合的特异性、生物相容性荧光传感器，用于 HSA 检测
DOI：
10.1016/j.bios.2016.07.002
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发表时间：
2016
影响因子：
12.6
通讯作者：
Xiao Dan
中科院分区：
文献类型：
--
作者：
Liao Caiyun;Li Fangfang;Huang Shanshan;Zheng Baozhan;Du Juan;Xiao Dan
针对血清中含有大量蛋白质、小分子和离子的特定分析物，设计无毒和选择性的传感器仍然是临床诊断的挑战。本文报道了一种合成的绿色荧光蛋白（GFP）生色团与多肽的杂交体H1，该杂交体具有明显的荧光增强作用，可用于人血清白蛋白（HSA）的检测。荧光反应是由H1与HSA的IIA亚结构域空腔之间的非共价结合引起的，这种特异性结合行为可以通过位于同一亚结构域的唯一色氨酸残基（Trp-214）的荧光强度的降低来证实。H1对HSA具有很高的敏感性和选择性，不受其他蛋白的干扰。H1的荧光强度与HSA浓度在0 ~ 67.0 μg mL− 1范围内呈良好的线性关系，检测限可达198.6 ng L−1。H1还成功地用于检测健康人尿液和血清中的微量HSA。所得H1/HSA体系还显示出令人满意的对阴离子表面活性剂十二烷基硫酸钠（SDS）的传感能力，通过降低荧光强度，检测限为3.48 ng L−1。
It is still a challenge for clinical diagnostics to design non-toxic and selective sensors for a specific analyte in blood serum, which contains a large number of proteins, small molecules, and ions. Herein, H1, a synthetic hybrid of green fluorescent protein (GFP) chromophore and peptide for human serum albumin (HSA) detection via obvious fluorescence enhancement was reported. The fluorescence response was caused by the non-covalent binding between H1 and the subdomain IIA cavity of HSA, and this specific binding behavior can be confirmed by the decrease of fluorescence intensity of the sole tryptophan residue (Trp-214) located in the same subdomain. H1 showed remarkable sensitivity and selectivity toward HSA without interference from other proteins. A good linear relationship between the fluorescence intensity of H1 and HSA concentration from 0 to 67.0 μg mL−1was obtained with the detection limit reaching as low as 198.6 ng L−1. Moreover, H1 was successfully utilized to detect trace HSA in healthy human urine (HU) and human blood serum (HBS), respectively. The resulting H1/HSA system also showed a satisfactory sensing ability toward anionic surfactant sodium dodecyl sulfate (SDS) through decreased fluorescence intensity with the detection limit of 3.48 ng L−1.