ACTIVATION OF THE T24 BLADDER-CARCINOMA TRANSFORMING GENE IS LINKED TO A SINGLE AMINO-ACID CHANGE
ACTIVATION OF THE T24 BLADDER-CARCINOMA TRANSFORMING GENE IS LINKED TO A SINGLE AMINO-ACID CHANGE
复制标题
DOI：
10.1038/300762a0
复制
发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
TAPAROWSKY, E;SUARD, Y;WIGLER, M
在人和鼠源性多种肿瘤和肿瘤细胞系的DNA中观察到几种不同的转化基因，这些基因可以诱导NIH3T3细胞1-5的形态转化。在人膀胱癌细胞系T24中发现的转化基因是H-ras-1，它是Harvey肉瘤病毒癌基因(v-H-ras)6-9的人类同源物。从T24 DNA中克隆的H-ras-1基因可以诱导NIH3T3细胞转化，而从正常细胞DNA中克隆的H-ras-1基因不能诱导NIH3T3细胞转化。转化基因和正常基因在功能上的显著区别似乎是单一碱基突变，这会导致基因编码的蛋白质序列中的氨基酸发生变化。
Several different transforming genes have been observed in the DNA of a variety of tumours and tumour cell lines of human and rodent origin by the ability of these genes to induce morphological transformation in NIH 3T3 cells1–5. The transforming gene found in a human bladder carcinoma cell line, T24, is H-ras-1, the human homologue of the Harvey sarcoma virus oncogene (v-H-ras)6–9. In the present study we have compared the H-ras-1 genes cloned from T24 and normal human DNA. The H-ras-1 gene cloned from T24 DNA induces transformation in NIH 3T3 cells, while the same gene cloned from normal cellular DNA does not. The functionally significant difference between the transforming and normal genes appears to be a single base mutation, which produces an amino acid change in the sequence of the proteins that the genes encode.