Effects of Hangeshashinto on the nasal physiological function: An in vitro study
Effects of Hangeshashinto on the nasal physiological function: An in vitro study
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Hangeshashinto 对鼻生理功能的影响：一项体外研究
DOI：
10.1016/j.anl.2020.08.012
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Yasuhiro Tanaka
中科院分区：
文献类型：
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作者：
Kosuke Tochigi;Kazuhiro Omura;Keisuke Miyashita;Satoshi Aoki;Nobuyoshi Otori;Yasuhiro Tanaka
Hangeshashinto是日本汉方药，用于治疗口腔粘膜炎和胃肠炎。Hangeshashinto具有广谱抗菌活性，抑制粘膜中前列腺素（PG）E2的产生，并具有改善炎症状况的能力。除了这些作用，因为cAMP，一个组成的Hangeshashinto，促进纤毛跳动，Hangeshashinto也可以改善鼻粘膜的生理功能，包括纤毛上皮细胞，但细节是未知的。从10只日本白色家兔的鼻中隔采集健康鼻粘膜切片，置于胶原培养皿中进行组织培养，并用两种不同浓度的Hangeshashinto溶液（1.0%，n = 10和2.5%，n = 10）和cAMP溶液（50μM，n=10和100 μM，n=10）或生理盐水（对照，n= 10）冲洗。结果1.0%苦参素组鼻粘膜纤毛频率在第3、7天较对照组明显升高（p <0.05），2.5%苦参素组鼻粘膜纤毛频率在第3、7天较对照组明显升高（p < 0.05），2.5%苦参素组鼻粘膜纤毛频率在第7天较对照组明显升高（p < 0.05）。100 µM cAMP组的CBF也观察到这种趋势，1.0%汉草散组和100 µM cAMP组在冲洗后1、3和7天的CBF之间未观察到显著差异（p> 0.05）。结论1.0%汉麻液可促进鼻黏膜纤毛功能，促进鼻黏膜生理功能，而不损伤纤毛上皮。在适当的浓度下，汉麻香托能改善鼻粘膜上皮的生理功能。
ObjectiveHangeshashintois a Japanese Kampo medicine applied for the treatment of oral mucositis and gastroenteritis.Hangeshashintoexhibits broad-spectrum antibacterial activity and suppresses prostaglandin (PG)E2 production in the mucosa and has the ability to improve the inflammatory condition. In addition to these effects, because cAMP, a composition ofHangeshashinto, facilitates ciliary beat,Hangeshashintocould also improve the physiological function of the nasal mucosa, consist of ciliated epithelium, but details were unknown.MethodsThis study was aimed to investigate the effects ofHangeshashintoon the nasal mucosa. Healthy nasal mucosal sections were collected from the nasal septum of ten Japanese white rabbits, placed in a collagen dish for tissue culture, and rinsed with two different concentrations ofHangeshashintosolution (1.0%,n= 10 and 2.5%,n= 10) and cAMP solution (50µM,n=10 and 100 µM,n=10) or saline (control,n= 10). Ciliary beat frequency (CBF) as a physiological function of the nasal mucosa was recorded at 1, 3 and 7 days after rinsing, and histological evaluation of epithelial damage was performed at 7 days after rinsing.ResultsCBF in the 1.0% but not in the 2.5%Hangeshashintogroup, increased at 3 and 7 days compared with that in the control group (p < 0.05). This trend was also observed in the CBF in the 100 µM cAMP group, significant difference was not observed between the CBF of the 1.0%Hangeshashintogroup and the 100 µM cAMP group at 1, 3 and 7 days after rinsing (p> 0.05). Histological score only in the 2.5%Hangeshashintogroup was lower than that in the control group (p< 0.05), while a significant decline was not observed in the other groups compared to that in the control group (p> 0.05).ConclusionOur results suggest that 1.0%Hangeshashintosolution facilitates the physiological function of the nasal mucosa by promoting ciliary functions without histological damage of cilia epithelium. When applied with the appropriate concentration,Hangeshashintocould have ability to improve the physiological functions of the nasal mucosal epithelium.