Clonal analysis and cell lineages of the vertebrate central nervous system.
Clonal analysis and cell lineages of the vertebrate central nervous system.
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脊椎动物中枢神经系统的克隆分析和细胞谱系。
DOI：
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发表时间：
1985
影响因子：
13.9
通讯作者：
M. Jacobson
中科院分区：
文献类型：
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作者：
M. Jacobson
在引入辣根过氧化物酶（HRP）作为青蛙胚胎细胞内谱系示踪剂之前，追踪脊椎动物中枢神经系统细胞谱系的进展甚微（Jacobson & Hirose 1978、1981，Hirose & Jacobson 1979）。概念上的弱点和缺乏足够的技术相互促进，导致进展缓慢（Jacobson 1978，p.98）。因此，传统上对具有“马赛克”发育的胚胎和具有“调节”发育的胚胎进行了区分，在“马赛克”发育中，谱系决定了细胞命运，而在“调节”发育中，细胞相互作用决定了细胞命运，但事实上，大多数（如果不是全部）胚胎都表现出两者的组合（Harrison 1933，Weiss 1939，Davidson 1976）。我建议有几种不同自主程度的谱系模式。除了确定血统模式之外。它是完全自主的，其中给定的祖细胞与其后代的表型之间的关系是全部或没有，还有其他称为不确定谱系模式，其中祖先细胞与其后代的表型之间的关系在某种程度上取决于外部条件。现在已经清楚，细胞相互作用可以导致 Uro 和 Cephalo 脊索动物细胞命运的变化（Tung 等人，11958，1960；Nakauchi 和 Takashita，1983），其中有严格的证据表明，位于卵中并差异分布到不同谱系的物质决定了分化细胞的最终命运（Whittaker 1973，1979。Jeffery 1983，杰弗里等人 1983）。这种关于嵌合胚胎广泛调控的无懈可击的证据应该有助于打开那些不愿承认这一决心的人的思想
Very little progress in tracing cell lineages in the CNS of vertebrates had been made before the introduction of horseradish peroxidase (HRP) as an intra cellular lineage tracer in the frog embryo (Jacobson & Hirose 1978, 1981, Hirose & Jacobson 1979). Conceptual weaknesses and lack of adequate tech niques reinforced one another to ensure slow progress (Jacobson 1978, p. 98). Thus, a distinction has traditionally been made between embryos that have "mosaic" development, in which lineage determines cell fate, and those with "regulative" development, in which cell interactions determine cell fate, but, in fact, most if not all embryos show a combination of both (Harrison 1933, Weiss 1939, Davidson 1976). I shall propose that there are several lineage modes with different degrees of autonomy. In addition to the determinate lineage mode. which is completely autonomous and in which the relationship between a given progenitor and the phenotype(s) of its descendants is all-or none, there are others called indeterminant lineage modes in which the rela tionship between ancestral cells and the phenotypes of their descendants is contingent on external conditions to some degree. It is now clear that cell interactions can result in changes in of cellular fates in Uroand Cephalo chordates (Tung et a11958, 1960, Nakauchi & Takashita 1983), in which there is rigorous proof that materials localized in the egg and differentially distrib uted into different lineages determine the ultimate fates of the differentiated cells (Whittaker 1973, 1979. Jeffery 1983, Jeffery et al 1983). Such unim peachable evidence of extensive regulation in mosaic embryos should help to open the minds of those who have been unwilling to admit that determination