Expression of multiple forms of clusterin during light-induced retinal degeneration.
Expression of multiple forms of clusterin during light-induced retinal degeneration.
复制标题
光诱导的视网膜变性期间多种形式的簇蛋白的表达。
DOI：
10.1076/ceyr.23.3.157.5463
复制
发表时间：
2001
影响因子：
2
通讯作者：
Chader,G
中科院分区：
文献类型：
--
作者：
Wong,P;Ulyanova,T;Organisciak,DT;Bennett,S;Lakins,J;Arnold,JM;Kutty,RK;Tenniswood,M;vanVeen,T;Darrow,RM;Chader,G
PurposeClusterin has been associated with active cell death in several different model systems, including animal models of retinal degeneration. Clusterin is also expressed in normal tissues, a finding that leads to the question of how it could then play a cell death-specific role during tissue regression. To address this paradox, we have examined clusterin expression during light-induced retinal damage in rats.MethodsNormal albino rats were reared in darkness and then exposed to intense visible light to induce retinal degeneration. Clusterin expression was then examined at various times after light treatment. Standard molecular techniques including Northern analysis, immunohistochemistry, and Western analysis were employed.ResultsNorthern analysis established that the largest increase in clusterin expression occurs after a decrease in interphotoreceptor retinoid binding protein, IRBP, expression (an indication of a photoreceptor cell dysfunction) and after an increase in heme oxygenase 1, HO-1, expression (an oxidative stress inducible gene), suggesting that induction of clusterin expression is an oxidative stress response. Immunohistochemical analysis with two different clusterin-specific antibodies, anti(SGP-2) and anti(301), localized distinct forms of clusterin to Müller cells and degenerating photo-receptor cells. Western analysis demonstrated degeneration associated isoforms of clusterin in light treated retina that are not present in normal retina.ConclusionClusterin over-expression is characteristic of a retinal degeneration phenotype and we propose that clusterin action may be defined by the nature in which it is modified. We hypothesize that alternate processing leads to retinal degeneration-specific forms of the protein (65, 61, and 50 kDa) that are not present in normal retina.
